Department of Pharmacognosy Science, College of Pharmacy, Yan-Bian University, Yanji, Jilin Province 133000, China.
Nat Prod Res. 2012;26(16):1483-91. doi: 10.1080/14786419.2011.565473. Epub 2011 Oct 18.
Artemisia sacrorum Ledeb. was extracted by 95% ethanol and water, respectively. By partitioning the 95% ethanol extract successively with different solvents and separating the water extract by macroporous resin, nine separate parts were obtained. According to the results of in vitro experiments, the CH₂Cl₂ (dichloromethane) fraction showed the most pronounced cytotoxic activity against HepG2, HT-29 and MCF-7 cells, with EC₅₀ values 122.35, 49.76 and 28.51 µg mL⁻¹, respectively, at 48 h. Following this, the compounds of the CH₂Cl₂ fraction were separated and identified. Ten compounds were isolated from A. sacrorum Ledeb. and identified by spectral analysis. Four compounds, including acacetin, were isolated for the first time from A. sacrorum Ledeb.
采用 95%乙醇和水分别对青蒿进行提取，将 95%乙醇提取物依次用不同溶剂萃取，水提取物用大孔树脂进行分离，得到 9 个部位。根据体外实验结果，二氯甲烷（CH₂Cl₂）部位对 HepG2、HT-29 和 MCF-7 细胞的细胞毒性活性最强，在 48 h 时 EC₅₀ 值分别为 122.35、49.76 和 28.51 µg/mL。随后对 CH₂Cl₂部位的化合物进行了分离和鉴定。从青蒿中分离得到 10 个化合物，并通过光谱分析进行鉴定。其中，包括 acacetin 在内的 4 个化合物为首次从青蒿中分离得到。
