评价蜱传脑炎（TBE）患者血清和脑脊液中 CXCL10、CXCL11、CXCL12 和 CXCL13 趋化因子。
Evaluation of CXCL10, CXCL11, CXCL12 and CXCL13 chemokines in serum and cerebrospinal fluid in patients with tick borne encephalitis (TBE).
机构信息
Department of Infectious Diseases and Neuroinfections, Medical University of Bialystok, Bialystok, Poland.
出版信息
Adv Med Sci. 2011;56(2):311-7. doi: 10.2478/v10039-011-0033-z.
PURPOSE
The aim of the study was to assess the concentration of chemokines: CXCL10, XCL11, CXCL12, CXCL13 in serum and cerebrospinal fluid (CSF) in patients with tick-borne encephalitis (TBE) before and after treatment. We evaluated also the usefulness of these molecules in diagnosis and monitoring of inflammation in TBE.
METHODS
Twenty three patients hospitalized in The Department of Infectious Diseases and Neuroinfections of Medical University in Białystok, Poland were included in the study. Patients were divided into 2 groups: TBE group-patients with confirmed TBE and control group (CG): patients with excluded TBE and other inflammatory diseases of CNS. Concentration of CXCL10/IP-10, CXCL11/I-TAC, CXCL12/SDF-1α, CXCL13/BLC/BCA-1 in serum and CSF were measured with ELISA kits (R&D Systems, USA) according to the protocols.
RESULTS
The analysis of chemokines concentration in TBE patients before treatment and control group using ROC showed that serum CXCL10 and CXCL13 and CSF CXCL10, CXCL11, CXCL12 and CXCL13 differentiate both groups (p<0.05). The analysis of CXCL10, CXCL11, CXCL12 and CXCL13 before and after treatment showed that CXCL10 and CXCL11 in CSF and CXCL13 in serum differentiates both groups with p<0.05.
CONCLUSIONS
Concentration of CSF CXCL10, CXCL11, CXCL12, CXCL13 and serum CXCL10, CXCL13 may be good biomarkers of CNS inflammation caused by TBEV. Moreover concentration of CXCL10 in CSF and CXCL13 in serum may be used as indicators of patients recovery.
目的
本研究旨在评估蜱传脑炎（TBE）患者治疗前后血清和脑脊液（CSF）中趋化因子 CXCL10、XCL11、CXCL12 和 CXCL13 的浓度，并评估这些分子在 TBE 诊断和炎症监测中的作用。
方法
本研究纳入了 23 名在波兰比亚韦斯托克医科大学传染病和神经感染科住院的患者。患者被分为 2 组：TBE 组-确诊为 TBE 的患者和对照组（CG）：排除 TBE 和其他中枢神经系统炎症性疾病的患者。使用 ELISA 试剂盒（R&D Systems，美国）按照方案测量血清和 CSF 中 CXCL10/IP-10、CXCL11/I-TAC、CXCL12/SDF-1α、CXCL13/BLC/BCA-1 的浓度。
结果
使用 ROC 对治疗前 TBE 患者和对照组的趋化因子浓度进行分析表明，血清 CXCL10 和 CXCL13 以及 CSF CXCL10、CXCL11、CXCL12 和 CXCL13 可区分两组（p<0.05）。对治疗前后 CXCL10、CXCL11、CXCL12 和 CXCL13 的分析表明，CSF 中 CXCL10 和 CXCL11 以及血清中 CXCL13 可区分两组，差异具有统计学意义（p<0.05）。
结论
CSF CXCL10、CXCL11、CXCL12、CXCL13 和血清 CXCL10、CXCL13 的浓度可能是 TBEV 引起中枢神经系统炎症的良好生物标志物。此外，CSF 中 CXCL10 的浓度和血清中 CXCL13 的浓度可能可用作患者恢复的指标。
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