Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail), UMR_S 1085, F-35000 Rennes, France; NG Biotech, 35480 Guipry-Messac, France.
Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail), UMR_S 1085, F-35000 Rennes, France; Univ Rennes, CHU Rennes, F-35033 Rennes, France.
Cytokine. 2019 May;117:72-78. doi: 10.1016/j.cyto.2019.02.006. Epub 2019 Feb 28.
BACKGROUND & AIMS: The chemokines CXCL10 (interferon ϒ-inducible protein 10 [IP-10]), CXCL11 (Human interferon inducible T cell alpha chemokine [I-TAC]), and CXCL12 (stromal cell derived factor 1 [SDF-1]) contribute to cell recruitment, migration, activation, and homing in liver diseases and their serum levels have been shown to be associated with the degree of liver inflammation or fibrosis in various etiologies. However, the data may be contradictory or insufficient, particularly for CXCL12, in the field of chronic HCV infection. Here, we aimed to provide evidence for these chemokines as biomarkers for chronic HCV infection.
We analyzed the serum concentration of the three chemokines in healthy donors (n = 39) and patients (n = 87) with chronic HCV infection. Chemokine serum levels were compared to the stage of liver inflammation and fibrosis obtained from liver biopsies.
Serum CXCL10 and CXCL11 levels were higher at advanced stages of liver inflammation than at earlier stages, but the results were only of medium significance. Both serum CXCL11 and CXCL12 levels were significantly higher in cirrhotic patients than those with low or medium stages of fibrosis. The AUROCs were 0.8167 and 0.8574, respectively, for the diagnosis of cirrhotic patients.
These data provide evidence for the value of CXCL10, CXCL11, and CXCL12 as biomarkers of liver inflammation and fibrosis during chronic HCV infection. Serum CXCL10 and CXCL11 levels were associated with liver inflammation, but the level of significance was insufficient. However, serum CXCL11 and CXCL12 levels were elevated in cirrhotic patients, showing equivalent diagnostic accuracy as the existing established single serum fibrosis markers or algorithms.
趋化因子 CXCL10（干扰素 γ 诱导蛋白 10[IP-10]）、CXCL11（人干扰素诱导的 T 细胞α趋化因子[I-TAC]）和 CXCL12（基质细胞衍生因子 1[SDF-1]）有助于肝脏疾病中的细胞募集、迁移、激活和归巢，其血清水平已被证明与各种病因的肝炎症或纤维化程度相关。然而，在慢性 HCV 感染领域，这些数据可能存在矛盾或不足，特别是对于 CXCL12。在此，我们旨在为这些趋化因子作为慢性 HCV 感染的生物标志物提供证据。
我们分析了健康供体（n=39）和慢性 HCV 感染患者（n=87）的血清中这三种趋化因子的浓度。将趋化因子血清水平与肝活检获得的肝炎症和纤维化分期进行比较。
与早期炎症阶段相比，晚期炎症阶段的血清 CXCL10 和 CXCL11 水平更高，但结果仅具有中等意义。肝硬化患者的血清 CXCL11 和 CXCL12 水平均显著高于纤维化程度低或中等级别的患者。诊断肝硬化患者的 AUROCs 分别为 0.8167 和 0.8574。
这些数据为 CXCL10、CXCL11 和 CXCL12 作为慢性 HCV 感染期间肝炎症和纤维化的生物标志物的价值提供了证据。血清 CXCL10 和 CXCL11 水平与肝炎症相关，但显著性不足。然而，肝硬化患者的血清 CXCL11 和 CXCL12 水平升高，与现有的单一血清纤维化标志物或算法具有相当的诊断准确性。
