Ding Wenwen, Weng Huanjiao, Du Guocheng, Chen Jian, Kang Zhen
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
J Ind Microbiol Biotechnol. 2017 Aug;44(8):1127-1135. doi: 10.1007/s10295-017-1940-1. Epub 2017 Apr 5.
5-Aminolevulinic acid (ALA), the first committed intermediate for natural biosynthesis of tetrapyrrole compounds, has recently drawn intensive attention due to its broad potential applications. In this study, we describe the construction of recombinant Escherichia coli strains for ALA production from glucose via the C4 pathway. The hemA gene from Rhodobacter capsulatus was optimally overexpressed using a ribosome binding site engineering strategy, which enhanced ALA production substantially from 20 to 689 mg/L. Following optimization of biosynthesis pathways towards coenzyme A and precursor (glycine and succinyl-CoA), and downregulation of hemB expression, the production of ALA was further increased to 2.81 g/L in batch-fermentation.
5-氨基乙酰丙酸（ALA）是四吡咯化合物天然生物合成的首个关键中间体，由于其广泛的潜在应用，近来受到了广泛关注。在本研究中，我们描述了通过C4途径构建用于从葡萄糖生产ALA的重组大肠杆菌菌株。使用核糖体结合位点工程策略对来自荚膜红细菌的hemA基因进行了优化过表达，这使得ALA产量从20毫克/升大幅提高到689毫克/升。在对辅酶A和前体（甘氨酸和琥珀酰辅酶A）的生物合成途径进行优化以及下调hemB表达后，分批发酵中ALA产量进一步提高到2.81克/升。
