基于茎环 RT-PCR 的外周血中一组特定 microRNAs 作为转移性非小细胞肺癌的生物标志物。
A cluster of specified microRNAs in peripheral blood as biomarkers for metastatic non-small-cell lung cancer by stem-loop RT-PCR.
机构信息
Oncology Department, Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
出版信息
J Cancer Res Clin Oncol. 2012 Jan;138(1):85-93. doi: 10.1007/s00432-011-1068-z. Epub 2011 Oct 19.
Abstract
PURPOSE
To investigate the levels for some specified microRNAs in human's peripheral blood so as to determine whether they can serve as biomarkers for metastatic non-small-cell lung cancer.
METHODS
Use a quantitative stem-loop RT-PCR method to examine the serum levels for certain microRNAs including has-miR-125a-5p, has-miR-126, has-miR-183, has-miR-200, has-miR-221, and has-miR-222 from the patients with Stage IV, Stage I/II non-small-cell lung cancer and the controls.
RESULTS
There was statistical difference in the serum levels for hsa-miR-126, hsa-miR-183, and hsa-miR-222 between the controls and the Stage IV patients, but not for has-miR-125a-5p, has-miR-200 and has-miR-221. It also showed statistical difference for hsa-miR-126 and hsa-miR-183 between the Stage I/II patients and Stage IV patients, but not between the controls and Stage I/II patients.
CONCLUSION
Hsa-miR-126 and hsa-miR-183 may serve as potential serum biomarkers for metastatic non-small-cell lung cancer.
摘要
目的
检测人外周血中某些特定 microRNAs 的水平，以确定它们是否可作为转移性非小细胞肺癌的生物标志物。
方法
采用定量茎环 RT-PCR 方法检测血清中特定 microRNAs（包括 has-miR-125a-5p、has-miR-126、has-miR-183、has-miR-200、has-miR-221 和 has-miR-222）的水平，检测对象为 IV 期、I/II 期非小细胞肺癌患者和对照组人群。
结果
对照组与 IV 期患者的血清 hsa-miR-126、hsa-miR-183 和 hsa-miR-222 水平存在统计学差异，但 has-miR-125a-5p、has-miR-200 和 has-miR-221 水平不存在统计学差异。I/II 期患者与 IV 期患者的 hsa-miR-126 和 hsa-miR-183 水平也存在统计学差异，但对照组与 I/II 期患者的 hsa-miR-126 和 hsa-miR-183 水平不存在统计学差异。
结论
hsa-miR-126 和 hsa-miR-183 可能成为转移性非小细胞肺癌的潜在血清生物标志物。
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1
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PLoS One. 2011;6(6):e20351. doi: 10.1371/journal.pone.0020351. Epub 2011 Jun 7.
2
Plasma microRNAs as potential biomarkers for non-small-cell lung cancer.血浆 microRNAs 作为非小细胞肺癌的潜在生物标志物。
Lab Invest. 2011 Apr;91(4):579-87. doi: 10.1038/labinvest.2010.194. Epub 2010 Nov 29.
3
miR-200 Inhibits lung adenocarcinoma cell invasion and metastasis by targeting Flt1/VEGFR1.miR-200 通过靶向 Flt1/VEGFR1 抑制肺腺癌细胞的侵袭和转移。
Mol Cancer Res. 2011 Jan;9(1):25-35. doi: 10.1158/1541-7786.MCR-10-0497. Epub 2010 Nov 29.
4
The role of microRNA in human lung squamous cell carcinoma.微小RNA在人肺鳞状细胞癌中的作用。
Cancer Genet Cytogenet. 2010 Jul 15;200(2):127-33. doi: 10.1016/j.cancergencyto.2010.03.014.
5
microRNAs and lung cancer: tumors and 22-mers.微小 RNA 与肺癌：肿瘤与 22 个核苷酸。
Cancer Metastasis Rev. 2010 Mar;29(1):109-22. doi: 10.1007/s10555-010-9204-9.
6
Accurate classification of non-small cell lung carcinoma using a novel microRNA-based approach.基于新型 microRNA 方法的非小细胞肺癌精确分类。
Clin Cancer Res. 2010 Jan 15;16(2):610-9. doi: 10.1158/1078-0432.CCR-09-2638. Epub 2010 Jan 12.
7
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8
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9
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10
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