Albe K R, Butler M H, Wright B E
Microbiology Department, University of Montana, Missoula 59812.
J Theor Biol. 1990 Mar 22;143(2):163-95. doi: 10.1016/s0022-5193(05)80266-8.
The activity of crude and pure enzyme preparations as well as the molecular weight of these enzymes were obtained from the literature for several organisms. From these data enzyme concentrations were calculated and compared to the concentration(s) of their substrates in the same organism. The data are expressed as molar ratios of metabolite concentration to enzyme site concentration. Of the 140 ratios calculated, 88% were one or greater, indicating that in general substrates exceed their cognate enzyme concentrations. Of the 17 cases where enzyme exceeds metabolite concentration, 16 were in glycolysis. The data in general justify the use of enzyme kinetic mechanisms determined in vitro in the construction of dynamic models which simulate in vivo metabolism.
从文献中获取了几种生物体的粗酶制剂和纯酶制剂的活性以及这些酶的分子量。根据这些数据计算出酶浓度，并将其与同一生物体中底物的浓度进行比较。数据以代谢物浓度与酶位点浓度的摩尔比表示。在计算出的140个比率中，88%为1或更大，这表明一般来说底物超过了其同源酶的浓度。在酶超过代谢物浓度的17个案例中，有16个发生在糖酵解过程中。总体而言，这些数据证明了在构建模拟体内代谢的动态模型时，使用体外确定的酶动力学机制是合理的。
