使用脱钙骨基质的骨髓基质细胞修复大段骨缺损。
Repair of large segmental bone defects using bone marrow stromal cells with demineralized bone matrix.
机构信息
Department of Orthopaedics, Southwest Hospital, Tissue Engineering Center of the Third Military Medical University, Chongqing, China.
出版信息
Orthop Surg. 2009 Feb;1(1):34-41. doi: 10.1111/j.2757-7861.2008.00007.x.
OBJECTIVE
The aim of the present study was to evaluate the effect of tissue-engineered constructs on repair of large segmental bone defects in goats.
METHODS
Allogenic demineralized bone matrix (aDBM) was seeded with autologous marrow stromal cells (aMSC) for seven days to construct DBM-MSC grafts prior to implantation. 24 goats were randomly divided into three groups (eight in each). In each group, 3 cm diaphyseal femoral defects were created unilaterally, and subsequently filled with the DBM-MSC grafts, DBM alone and an untreated control, respectively. Radiological analysis and biomechanical evaluation were performed at 12 and 24 weeks after operation.
RESULTS
Obvious increases in radiological scoring and biomechanical strength were found in the DBM-MSC group when compared to the DBM group. X-ray examination showed excellent bone healing in the DBM-MSC group, whereas only partial bone repair was seen in the DBM group, and no healing in untreated controls. Histologically, a tendency to bone regeneration and remodeling was far more obvious for the DBM-MSC group than the DBM only and untreated controls.
CONCLUSION
Our results strongly suggest that transplantation of bone MSC within a DBM could have advantages for the bone repair of large segmental defects.
目的
本研究旨在评估组织工程构建物对山羊大节段骨缺损修复的影响。
方法
异体脱矿骨基质（aDBM）接种自体骨髓基质细胞（aMSC）7 天，构建 DBM-MSC 移植物，然后进行植入。24 只山羊随机分为三组（每组 8 只）。每组均在一侧造成 3cm 骨干缺损，分别用 DBM-MSC 移植物、DBM 单独和未处理的对照组填充。术后 12 和 24 周进行放射学分析和生物力学评估。
结果
与 DBM 组相比，DBM-MSC 组的放射学评分和生物力学强度明显增加。X 射线检查显示 DBM-MSC 组骨愈合良好，而 DBM 组仅部分骨修复，未处理的对照组无愈合。组织学上，DBM-MSC 组的骨再生和重塑趋势明显优于 DBM 组和未处理的对照组。
结论
我们的结果强烈表明，DBM 内移植骨 MSC 可能有利于大节段骨缺损的修复。
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