University of Sfax, Animal Physiology Laboratory, Life Sciences Department, Sfax Faculty of Science, BP 1171, 3000 Sfax, Tunisia.
Ecotoxicol Environ Saf. 2011 May;74(4):643-9. doi: 10.1016/j.ecoenv.2010.10.010. Epub 2010 Nov 12.
The present study investigates the bone maturity of suckling rats whose mothers were treated with gibberellic acid (GA(3)). Female Wistar rats were divided into two groups: group I that served as controls and group II that received orally GA(3) (200 ppm) from the 14th day of pregnancy until day 14 after delivery. In the GA(3) group, an increase in body and femur weights as well as in femur length of pups was noted when compared to controls. Lipid peroxidation was demonstrated by high femur malondialdehyde levels, while superoxide dismutase, catalase, glutathione peroxidase activities, glutathione and vitamin C levels in femur decreased. GA(3) caused a decrease in calcium and phosphorus levels in bone. The calcium concentration in plasma increased and the phosphorus concentration decreased, while urinary levels of calcium decreased and those of phosphate increased. Moreover, plasma total tartrate-resistant acid phosphatase and total alkaline phosphatase increased. Bone disorders were confirmed by femur histological changes.
本研究调查了母亲接受赤霉素（GA(3)）治疗的哺乳期大鼠的骨成熟度。雌性 Wistar 大鼠分为两组：对照组（I 组）和接受 GA(3)（200ppm）口服治疗组（从妊娠第 14 天至分娩后第 14 天）（II 组）。与对照组相比，GA(3)组的幼鼠体重、股骨重量和股骨长度均增加。股骨丙二醛水平升高表明脂质过氧化，而超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性、谷胱甘肽和维生素 C 水平降低。GA(3)导致骨中钙和磷水平降低。血浆中钙浓度增加，磷浓度降低，尿中钙浓度降低，磷酸盐浓度升高。此外，血浆总抗酒石酸酸性磷酸酶和总碱性磷酸酶增加。股骨组织学变化证实了骨疾病的存在。
