Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts 02142, USA.
Genome Res. 2012 Feb;22(2):292-8. doi: 10.1101/gr.126573.111. Epub 2011 Oct 18.
The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.
结直肠癌的肿瘤微环境是一个由基因组改变的癌细胞、非肿瘤细胞和多种微生物组成的复杂群落。这些成分中的每一个都可能促成癌变；然而，微生物组的作用是最不为人所知的。我们使用来自 9 对肿瘤/正常样本的全基因组序列，对结直肠癌中的微生物组组成进行了描述。通过对 95 对癌/正常 DNA 样本的定量 PCR 和 16S rDNA 序列分析，确认了梭杆菌序列在癌组织中富集，而拟杆菌门和厚壁菌门在肿瘤中减少。使用 FISH 也在结直肠肿瘤中观察到了梭杆菌。这些发现揭示了结直肠癌微生物组的改变；然而，梭杆菌在结直肠癌发病机制中的确切作用需要进一步研究。
