Department of GI Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University School of Medicine, No.301 Yanchang Road, Zhabei District, Shanghai, China.
Research Institute of Intestinal Diseases, Tongji University School of Medicine, Shanghai, China.
Eur J Clin Microbiol Infect Dis. 2017 Nov;36(11):2073-2083. doi: 10.1007/s10096-017-3026-4. Epub 2017 Jun 9.
The aim of this study was to explore the gut microbiota profiles of colorectal cancer (CRC) patients and to examine the relationship between gut microbiota and other key molecular factors involved in CRC tumorigenesis. In this study, a 16S rDNA sequencing platform was used to identify possible differences in the microbiota signature between CRC and adjacent normal mucosal tissue. Differences in the microbiota composition in different anatomical colorectal tumor sites and their potential association with KRAS mutation were also explored. In this study, the number of Firmicutes and Actinobacteria decreased, while the number of Fusobacteria increased in the gut of CRC patients. In addition, at the genus level, Fusobacterium was identified as the key contributor to CRC tumorigenesis. In addition, a different distribution of gut microbiota in ascending and descending colon cancer samples was observed. Lipopolysaccharide biosynthesis-associated microbial genes were enriched in tumor tissues. Our study suggests that specific mucosa-associated microbiota signature and function are significantly changed in the gut of CRC patients, which may provide insight into the progression of CRC. These findings could also be of value in the creation of new prevention and treatment strategies for this type of cancer.
本研究旨在探讨结直肠癌（CRC）患者的肠道微生物群特征，并研究肠道微生物群与 CRC 肿瘤发生过程中涉及的其他关键分子因素之间的关系。在本研究中，使用 16S rDNA 测序平台来鉴定 CRC 和相邻正常黏膜组织之间微生物群特征的可能差异。还探索了不同解剖学结直肠肿瘤部位的微生物群落组成的差异及其与 KRAS 突变的潜在关联。在本研究中，CRC 患者肠道中的厚壁菌门和放线菌门数量减少，而梭杆菌门数量增加。此外，在属水平上，梭杆菌属被确定为 CRC 肿瘤发生的关键贡献者。此外，还观察到升结肠癌和降结肠癌样本中肠道微生物群的不同分布。与脂多糖生物合成相关的微生物基因在肿瘤组织中富集。我们的研究表明，CRC 患者肠道中特定的黏膜相关微生物群特征和功能发生了显著变化，这可能为 CRC 的进展提供新的见解。这些发现对于开发针对这种癌症的新的预防和治疗策略也可能具有重要价值。
