Department of Oral and Maxillofacial Surgery, Hamamatsu Kita Hospital, Hamamatsu, Japan.
Int J Oral Maxillofac Implants. 2011 Sep-Oct;26(5):947-54.
The aim of this comparative study was to investigate cell-based effective bone engineering and the correlation between the osseointegration of dental implants and tissue-engineered bone using dental pulp stem cells (DPSC), bone marrow stem cells (BMSC), and periosteal cells (PC).
The first molar and all premolars were extracted from the mandibles of three dogs, and in each dog, six bone defects (three on each side) were prepared with a 10-mm-diameter trephine bur after 4 weeks. Different materials were implanted in the defects and the sites were allowed to heal. The experimental groups were as follows: (1) dog DPSC and platelet-rich plasma (PRP) (dDPSC/PRP), (2) dog BMSC and PRP (dBMSC/PRP), (3) dog PC and PRP (dPC/PRP), and (4) control (defect only). Eight weeks later, dental implants were placed in the defects. After another 8 weeks, the amount of bone regeneration was assessed by histologic and histomorphometric analyses (bone-implant contact).
The mean bone-implant contact values were 66.7% ± 3.6% for group 1 (dDPSC/PRP), 62.5% ± 3.1% for group 2 (dBMSC/PRP), 39.4% ± 2.4% for group 3 (dPC/PRP), and 30.3% ± 2.6% for the control group.
DPSC showed the highest osteogenic potential and may be a useful cell source for tissue-engineered bone around dental implants.
本对比研究旨在探讨基于细胞的有效骨工程以及牙髓干细胞（DPSC）、骨髓干细胞（BMSC）和骨膜细胞（PC）用于牙种植体骨整合的组织工程骨之间的相关性。
从 3 只狗的下颌骨中取出第一磨牙和所有前磨牙，在每只狗中，在 4 周后使用 10mm 直径的环钻制备 6 个骨缺损（每侧 3 个）。将不同的材料植入缺损部位并进行愈合。实验组如下：（1）狗 DPSC 和富血小板血浆（PRP）（dDPSC/PRP），（2）狗 BMSC 和 PRP（dBMSC/PRP），（3）狗 PC 和 PRP（dPC/PRP），和（4）对照组（仅缺损）。8 周后，在缺损部位植入牙种植体。8 周后，通过组织学和组织形态计量学分析（骨-种植体接触）评估骨再生量。
第 1 组（dDPSC/PRP）的平均骨-种植体接触值为 66.7%±3.6%，第 2 组（dBMSC/PRP）为 62.5%±3.1%，第 3 组（dPC/PRP）为 39.4%±2.4%，对照组为 30.3%±2.6%。
DPSC 显示出最高的成骨潜力，可能是牙种植体周围组织工程骨的有用细胞来源。
