Department of Biology, Faculty of Mathematical and Natural Science, University of Prishtina, Mother Theresa St, 10000 Prishtinë, Kosovo.
Nat Prod Res. 2012;26(18):1676-81. doi: 10.1080/14786419.2011.613384. Epub 2011 Oct 20.
Leaves and inflorescences of Stachys sylvatica L. (Lamiaceae) were collected from three different wild populations in Kosovo to study the natural variation of the chemical composition of essential oils, total flavonoids, total phenolics and the antioxidant activity. Essential oils were obtained by steam distillation and analysed by GC-FID and GC-MS, whereas total flavonoids, total phenolics and antioxidant activities were determined by spectrophotometric methods. Yields of essential oils ranged from 0.001% to 0.007% (v per dry weight). Twenty-eight volatile constituents were identified. The main constituents were α-pinene, β-pinene and germacrene-D. Total phenolics ranged from 39.3 to 70.8 mg g⁻¹ dry mass, whereas total flavonoid content ranged from 30.44 to 70.63 mg g⁻¹ dm. The antioxidant activity, as measured by the DPPH method, exhibited a rather high degree of activity ranging from 25.5% to 57.2%, whereas the FRAP antioxidant activity showed a lower variability and ranged from 93 to 133.4 mg g⁻¹ dm.
从科索沃的三个不同野生种群中采集了 Stachys sylvatica L.（唇形科）的叶和花序，以研究精油、总黄酮、总酚和抗氧化活性的化学成分的自然变异。通过水蒸气蒸馏获得精油，并通过 GC-FID 和 GC-MS 进行分析，而总黄酮、总酚和抗氧化活性则通过分光光度法测定。精油的产率范围为 0.001%至 0.007%（每干重的体积）。鉴定出 28 种挥发性成分。主要成分是α-蒎烯、β-蒎烯和大根香叶烯-D。总酚含量范围为 39.3 至 70.8mg/g 干质量，而总黄酮含量范围为 30.44 至 70.63mg/g 干质量。通过 DPPH 法测定的抗氧化活性表现出相当高的活性，范围为 25.5%至 57.2%，而 FRAP 抗氧化活性的变异性较低，范围为 93 至 133.4mg/g 干质量。
