Department of Endocrinology and Metabolism MEA, Aarhus University Hospital, Aarhus, Denmark.
Nutr J. 2011 Oct 19;10:115. doi: 10.1186/1475-2891-10-115.
Obesity is a state of chronic low-grade inflammation. Chronic low-grade inflammation is associated with the pathophysiology of both type-2 diabetes and atherosclerosis. Prevention or reduction of chronic low-grade inflammation may be advantageous in relation to obesity related co-morbidity. In this study we investigated the acute effect of dietary protein sources on postprandial low-grade inflammatory markers after a high-fat meal in obese non-diabetic subjects.
We conducted a randomized, acute clinical intervention study in a crossover design. We supplemented a fat rich mixed meal with one of four dietary proteins - cod protein, whey isolate, gluten or casein. 11 obese non-diabetic subjects (age: 40-68, BMI: 30.3-42.0 kg/m2) participated and blood samples were drawn in the 4 h postprandial period. Adiponectin was estimated by ELISA methods and cytokines were analyzed by multiplex assay.
MCP-1 and CCL5/RANTES displayed significant postprandial dynamics. CCL5/RANTES initially increased after all meals, but overall CCL5/RANTES incremental area under the curve (iAUC) was significantly lower after the whey meal compared with the cod and casein meals (P = 0.0053). MCP-1 was initially suppressed after all protein meals. However, the iAUC was significantly higher after whey meal compared to the cod and gluten meals (P = 0.04).
We have demonstrated acute differential effects on postprandial low grade inflammation of four dietary proteins in obese non-diabetic subjects. CCL5/RANTES initially increased after all meals but the smallest overall postprandial increase was observed after the whey meal. MCP-1 was initially suppressed after all 4 protein meals and the whey meal caused the smallest overall postprandial suppression.
ClinicalTrials.gov ID: NCT00863564.
肥胖是一种慢性低度炎症状态。慢性低度炎症与 2 型糖尿病和动脉粥样硬化的病理生理学有关。预防或减少慢性低度炎症可能有利于肥胖相关的合并症。在这项研究中，我们研究了在肥胖非糖尿病患者中，高脂肪餐后不同膳食蛋白质来源对餐后低度炎症标志物的急性影响。
我们采用随机、急性临床干预研究的交叉设计。我们用四种膳食蛋白质之一（鳕鱼蛋白、乳清分离蛋白、面筋或酪蛋白）补充富含脂肪的混合餐。11 名肥胖非糖尿病受试者（年龄：40-68 岁，BMI：30.3-42.0kg/m2）参与了研究，并在餐后 4 小时内抽取血样。采用 ELISA 方法测定脂联素，采用多重分析测定细胞因子。
MCP-1 和 CCL5/RANTES 显示出显著的餐后动力学。CCL5/RANTES 最初在所有餐后均增加，但与鳕鱼和酪蛋白餐相比，乳清餐后的 CCL5/RANTES 增量曲线下面积（iAUC）显著较低（P=0.0053）。所有蛋白质餐后 MCP-1 最初均受到抑制。然而，乳清餐后的 iAUC 明显高于鳕鱼和面筋餐后（P=0.04）。
我们已经证明，在肥胖非糖尿病受试者中，四种膳食蛋白质对餐后低度炎症有急性差异影响。CCL5/RANTES 最初在所有餐后均增加，但乳清餐后的总体餐后增加最小。所有 4 种蛋白质餐后 MCP-1 最初均受到抑制，而乳清餐引起的总体餐后抑制最小。
ClinicalTrials.gov 编号：NCT00863564。
