Ponomareva S I, Moroz B V
Zh Mikrobiol Epidemiol Immunobiol. 1990 May(5):85-9.
The results of comparative study of sera obtained from donors and from several groups of patients with suspected toxoplasmosis are presented. The study has been carried out with the use of commercial enzyme immunoassay (EIA) kits: Toxoplasma gondii IgG EIA and Toxoplasma gondii IgM EIA manufactured by Labsystems (Finland), Sevatest ELISA IgG Toxo Micro I manufactured by Sevac (Czechoslovakia). Statistical processing of the results has confirmed the identity of these kits. The necessity of using evaluation criteria (the separation point, the scale for the interpretation of results) when working with the Sevac kits is emphasized. Comparative evaluation of antibody profiles in the sera under test suggests that the titer less than 1:1600 should be regarded as the separation point for these kits. IgM antibodies to T. gondii have been found only in 22% of patients with high titers of IgG antibodies.
本文展示了从捐赠者以及几组疑似弓形虫病患者处获取的血清的对比研究结果。该研究使用了商用酶免疫测定(EIA)试剂盒:芬兰Labsystems公司生产的弓形虫IgG EIA试剂盒和弓形虫IgM EIA试剂盒,以及捷克斯洛伐克Sevac公司生产的Sevatest ELISA IgG Toxo Micro I试剂盒。结果的统计处理证实了这些试剂盒的一致性。强调了使用Sevac试剂盒时采用评估标准(分界点、结果解读范围)的必要性。对受试血清中抗体谱的对比评估表明,这些试剂盒的分界点应为滴度低于1:1600。仅在22%的高滴度IgG抗体患者中发现了弓形虫IgM抗体。