[用于检测弓形虫抗体的Sevatest商业ELISA免疫酶试剂盒与其他血清学检测方法的比较评估]

[Comparative evaluation of the commercial Sevatest ELISA immunoenzyme set and other serological tests for detecting Toxoplasma gondii antibodies].

作者信息

Moroz B V, Ponomareva S I, Mikheeva M N, Zhirova M V, Triakina I P

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1987 Feb(2):60-3.

Abstract

This work analyzes the results of 4 serologic tests used for the diagnosis of toxoplasmosis: the complement fixation (CFT), indirect immunofluorescence (IIF), passive hemagglutination (PHAT) tests, and the enzyme-linked immunosorbent assay (ELISA). The last-mentioned one was made with the use of the commercial kits Sevatest ELISA IgG/Toxo Micro I. The results of ELISA were in good correlation with those yielded by the traditional tests: 70% coincidence with CFT, 80% with IIF, 84% with PHAT; besides, ELISA has shown a higher sensitivity in the screening of sera.

摘要

本研究分析了用于诊断弓形虫病的4种血清学检测方法的结果：补体结合试验（CFT）、间接免疫荧光试验（IIF）、被动血凝试验（PHAT）和酶联免疫吸附测定（ELISA）。最后一种检测方法使用的是商业试剂盒Sevatest ELISA IgG/Toxo Micro I。ELISA的结果与传统检测方法的结果具有良好的相关性：与CFT的符合率为70%，与IIF的符合率为80%，与PHAT的符合率为84%；此外，ELISA在血清筛查中显示出更高的敏感性。