用于检测弓形虫IgG和IgM抗体的自动化微粒酶免疫测定法。
Automated microparticle enzyme immunoassays for IgG and IgM antibodies to Toxoplasma gondii.
作者信息
Safford J W, Abbott G G, Craine M C, MacDonald R G
机构信息
Diagnostic Division, Abbott Laboratories, North Chicago, Illinois 60064.
出版信息
J Clin Pathol. 1991 Mar;44(3):238-42. doi: 10.1136/jcp.44.3.238.
Fully automated microparticle enzyme immunoassays (MEIA) for the IMx immunoassay analyser were developed to detect IgG and IgM antibodies to Toxoplasma gondii. The IgG MEIA results are expressed in International Units (IU) of IgG antibody interpolated from a six point calibration curve covering the range from 0 to 300 IU/ml. Reproducible results were obtained from a calibration curve stored in the instrument for at least one month. The qualitative IgM MEIA expresses results as an index using a single calibrator included in each run. The Toxo IgG MEIA and Toxo IgM MEIA were in 98% and 97% agreement, respectively, with the reference assays used. Twenty four sera can be completely processed in about 35 minutes.
已开发出用于IMx免疫分析仪器的全自动微粒酶免疫测定法（MEIA），以检测抗弓形虫的IgG和IgM抗体。IgG MEIA结果以IgG抗体的国际单位（IU）表示，该单位通过覆盖0至300 IU/ml范围的六点校准曲线内插得出。从仪器中存储至少一个月的校准曲线可获得可重复的结果。定性IgM MEIA使用每次运行中包含的单个校准物将结果表示为一个指数。弓形虫IgG MEIA和弓形虫IgM MEIA分别与所使用的参考测定法有98%和97%的一致性。大约35分钟内可完全处理24份血清。
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