Pezzella F, Tse A G, Cordell J L, Pulford K A, Gatter K C, Mason D Y
Nuffield Department of Pathology, John Radcliffe Hospital, Headington, United Kingdom.
Am J Pathol. 1990 Aug;137(2):225-32.
It has been reported previously that the bcl-2 protooncogene protein is detectable in neoplastic cells from cases of human lymphoma in which the 14;18 chromosomal translocation is present, but not in lymphomas that lack this chromosomal rearrangement or in normal lymphoid tissue. In the present study we confirmed, by immunohistologic labeling with polyclonal and monoclonal antibodies, that bcl-2 protein is strongly expressed in many cases of follicular lymphoma and that these neoplastic follicles differ clearly from their nonmalignant counterpart (reactive germinal centres) in which bcl-2 protein is undetectable. However we also found bcl-2 protein in normal T- and B-lymphoid cells and in a variety of lymphoproliferative disorders in which the 14;18 translocation is not present. It is therefore concluded that expression of bcl-2 protein is not a specific marker for lymphomas bearing the 14;18 chromosomal translocation and that the observations of other investigators may have reflected the inadequate sensitivity of their staining procedure.
先前已有报道称，在存在14;18染色体易位的人类淋巴瘤病例的肿瘤细胞中可检测到bcl-2原癌基因蛋白，但在缺乏这种染色体重排的淋巴瘤或正常淋巴组织中则检测不到。在本研究中，我们通过使用多克隆和单克隆抗体进行免疫组织化学标记证实，bcl-2蛋白在许多滤泡性淋巴瘤病例中强烈表达，并且这些肿瘤性滤泡与未检测到bcl-2蛋白的非恶性对应物（反应性生发中心）明显不同。然而，我们也在正常T和B淋巴细胞以及不存在14;18易位的各种淋巴增殖性疾病中发现了bcl-2蛋白。因此得出结论，bcl-2蛋白的表达不是携带14;18染色体易位淋巴瘤的特异性标志物，其他研究者的观察结果可能反映了其染色程序的敏感性不足。
