通过培养和 16S rRNA 基因分析发现与囊性纤维化患者慢性肺部感染相关的真正微生物组。
True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis.
机构信息
Department of Biotechnology, Chemistry, and Environmental Engineering, Faculty of Engineering and Science, Aalborg University, Aalborg, Denmark.
出版信息
J Clin Microbiol. 2011 Dec;49(12):4352-5. doi: 10.1128/JCM.06092-11. Epub 2011 Oct 19.
Abstract
Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.
摘要
患有囊性纤维化 (CF) 的患者会发展为慢性肺部感染。在这项研究中，我们通过标准培养和 16S rRNA 基因分析研究了移植的 CF 肺中的微生物（n = 5）。培养和分子方法之间存在一致性。总之，标准培养似乎可用于鉴定主要病原体。
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