利用新型指示菌株肺炎克雷伯菌 ATCC 700603 对铜绿假单胞菌进行敏感且特异的改良 Hodge 试验，以检测 KPC 和金属β-内酰胺酶。
Sensitive and specific modified Hodge test for KPC and metallo-beta- lactamase detection in Pseudomonas aeruginosa by use of a novel indicator strain, Klebsiella pneumoniae ATCC 700603.
机构信息
Servicio Antimicrobianos, INEI-ANLIS Dr. Carlos G. Malbrán, Velez Sarsfield 563 Ave. (C1282AFF), Buenos Aires, Argentina.
出版信息
J Clin Microbiol. 2011 Dec;49(12):4301-3. doi: 10.1128/JCM.05602-11. Epub 2011 Oct 19.
Abstract
We evaluated the ability of the modified Hodge test to discriminate between KPC- and metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa isolates and carbapenemase nonproducers. With Escherichia coli ATCC 25922 as the indicator strain, the MHT resulted in low sensitivity, specificity, and repeatability. Replacing the indicator strain with Klebsiella pneumoniae ATCC 700603 led to an improved performance (100%, 97%, 0%, and 100% sensitivity, specificity, indeterminate results and repeatability, respectively).
摘要
我们评估了改良 Hodge 试验（MHT）区分产 KPC 和产金属β-内酰胺酶（MBL）铜绿假单胞菌分离株与碳青霉烯酶非产酶株的能力。以大肠埃希菌 ATCC 25922 作为指示菌株时，MHT 的灵敏度、特异性和可重复性均较低。用肺炎克雷伯菌 ATCC 700603 替代指示菌株可改善其性能（分别为 100%、97%、0%和 100%的灵敏度、特异性、不确定结果和可重复性）。
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本文引用的文献
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