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表型检测法用于检测超广谱β-内酰胺酶和碳青霉烯酶的评价：微生物学实验室手册。

Review of phenotypic assays for detection of extended-spectrum β-lactamases and carbapenemases: a microbiology laboratory bench guide.

机构信息

Department of Medical Microbiology, College of Health Sciences, Makerere University, Kampala, Uganda.

出版信息

Afr Health Sci. 2020 Sep;20(3):1090-1108. doi: 10.4314/ahs.v20i3.11.

DOI:10.4314/ahs.v20i3.11
PMID:33402954
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7751514/
Abstract

BACKGROUND

Infections caused by gram-negative antibiotic-resistant bacteria continue to increase. Despite recommendations by the Clinical Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) with regards to detection of antibiotic degrading enzymes secreted by these bacteria, the true prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase producers remains a difficult task to resolve. Describing of previously designed phenotypic detection assays for ESBLs and carbapenemases in a single document avails a summary that allows for multiple testing which increases the sensitivity and specificity of detection.

METHODS AND AIMS

This review, therefore, defined and classified ESBLs and carbapenemases, and also briefly described how the several previously designed phenotypic detection assays for the same should be performed.

CONCLUSION

Extended-spectrum β-lactamase and carbapenemase detection assays, once performed correctly, can precisely discriminate between bacteria producing these enzymes and those with other mechanisms of resistance to β-lactam antibiotics.

摘要

背景

由革兰氏阴性抗生素耐药菌引起的感染继续增加。尽管临床实验室标准化协会 (CLSI) 和抗菌药物敏感性检测欧洲委员会 (EUCAST) 就这些细菌分泌的抗生素降解酶的检测提出了建议，但要确定扩展型β-内酰胺酶 (ESBL) 和碳青霉烯酶产生菌的真实流行率仍然是一项艰巨的任务。在一份文件中描述先前设计的 ESBL 和碳青霉烯酶表型检测方法，可以提供一个便于进行多项检测的摘要，从而提高检测的敏感性和特异性。

方法和目的

因此，本综述定义和分类了 ESBL 和碳青霉烯酶，并简要描述了应如何进行几种先前设计的用于检测相同物质的表型检测方法。

结论

一旦正确进行，ESBL 和碳青霉烯酶检测试验可以精确区分产生这些酶的细菌与具有其他β-内酰胺类抗生素耐药机制的细菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/365334a03365/AFHS2003-1090Fig16.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/365334a03365/AFHS2003-1090Fig16.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/56026755c8d2/AFHS2003-1090Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/71451fa36e41/AFHS2003-1090Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/3de755a9db7e/AFHS2003-1090Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/4fc4afaab720/AFHS2003-1090Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/7c9996260356/AFHS2003-1090Fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/d08ae2c5de3d/AFHS2003-1090Fig12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/7938968957b5/AFHS2003-1090Fig13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39eb/7751514/1808937e6718/AFHS2003-1090Fig14.jpg
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Evaluation of three protocols for direct susceptibility testing for gram negative-Enterobacteriaceae from patient samples in Uganda with SMS reporting.乌干达采用 SMS 报告方式，评估三种方案对患者样本中的革兰氏阴性肠杆菌科进行直接药敏试验的效果。
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