Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
Mol Med Rep. 2012 Feb;5(2):410-4. doi: 10.3892/mmr.2011.640. Epub 2011 Oct 19.
Yes-associated protein (YAP) has been identified as an oncoprotein that regulates organ size by modulating proliferative and apoptotic activities. The aim of this study was to measure YAP gene expression and protein levels during rat liver regeneration and in liver cancer, and to explore possible correlations between YAP levels and cancer cell proliferation following partial hepatectomy. We examined YAP expression in rat regenerating liver using real-time PCR, Western blotting and immunohistochemical methods. YAP mRNA and protein levels were measured in human hepatocellular carcinoma. The results demonstrated that YAP protein levels were markedly increased in the regenerating liver (2.5-3 times), while YAP mRNA levels decreased slightly (1.47-2.17 times) (P=0.017). YAP mRNA and protein levels were both higher in hepatocellular carcinoma than in para-cancerous tissue. In conclusion, liver regeneration alone elevated YAP protein levels without elevating YAP mRNA levels. YAP mRNA and protein levels were both elevated in liver cancer cells.
Yes 相关蛋白 (YAP) 已被鉴定为一种癌蛋白，通过调节增殖和凋亡活性来调节器官大小。本研究的目的是测量大鼠肝再生和肝癌中 YAP 基因表达和蛋白水平，并探讨部分肝切除后 YAP 水平与癌细胞增殖之间的可能相关性。我们使用实时 PCR、Western blot 和免疫组织化学方法检查了大鼠再生肝中的 YAP 表达。测量了人肝癌中 YAP mRNA 和蛋白水平。结果表明，再生肝中 YAP 蛋白水平显著升高（2.5-3 倍），而 YAP mRNA 水平略有下降（1.47-2.17 倍）（P=0.017）。肝癌中 YAP mRNA 和蛋白水平均高于癌旁组织。总之，单独的肝再生会升高 YAP 蛋白水平而不会升高 YAP mRNA 水平。肝癌细胞中 YAP mRNA 和蛋白水平均升高。
