Carbonel Adriana Aparecida Ferraz, Simões Ricardo Santos, Santos Regiane Helena Barros Rabelo, Baracat Maria Cândida Pinheiro, Simões Manuel de Jesus, Baracat Edmund Chada, Soares Júnior José Maria
Departamento de Morfologia e Genética, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Rev Assoc Med Bras (1992). 2011 Sep-Oct;57(5):534-9. doi: 10.1590/s0104-42302011000500011.
To evaluate the effects of high-dose isoflavones on the uterus of castrated adult rats.
Adult, ovariectomized virgin rats (n = 40) were treated by gavage during 30 consecutive days with vehicle (propylene glycol, group GCtrl) or different doses of genistein: 42 (group GES42), 125 (GES125), or 250 (GES250) µg/g body weight per day. Animals were killed, weighed, vaginal and uterine samples were taken for cytologic evaluation, and serum levels of 17 β-estradiol and progesterone were determined. The middle third of the uterine horns was dissected, fixed in 10% formaldehyde and processed for paraffin inclusion; 5-µm thick sections were obtained and stained with HE for further histological study under light microscopy. The endometrial morphology and area, number and area of glands, and number of eosinophils in the lamina propria were analyzed. ANOVA and the Tukey-Kramer test were used for statistical analyses.
Uterine weight, endometrial glandular area, and number of glands and eosinophils were all higher in GES250 > G125 than in the other groups (GES250 > GES125 > GES42 = GCtrl; p < 0.05). Morphological data showed signs of endometrial proliferation upon treatment with genistein, especially in animals in GES125 and GES250 compared to other groups. In all animals in GES250, signs of uterine squamous metaplasia were observed.
A short treatment period with high daily doses of isoflavones can promote endometrial squamous metaplasia in ovariectomized rats.
评估高剂量异黄酮对去势成年大鼠子宫的影响。
成年未交配的去卵巢大鼠（n = 40）连续30天经口灌胃给予赋形剂（丙二醇，GCtrl组）或不同剂量的染料木黄酮：42（GES42组）、125（GES125组）或250（GES250组）μg/g体重/天。处死动物，称重，采集阴道和子宫样本进行细胞学评估，并测定血清17β-雌二醇和孕酮水平。分离子宫角的中间三分之一，固定于10%甲醛中，处理后包埋于石蜡；制成5μm厚的切片，用苏木精-伊红染色，在光学显微镜下进行进一步的组织学研究。分析子宫内膜形态和面积、腺体数量和面积以及固有层嗜酸性粒细胞数量。采用方差分析和Tukey-Kramer检验进行统计学分析。
GES250组和GES125组的子宫重量、子宫内膜腺体面积、腺体数量和嗜酸性粒细胞数量均高于其他组（GES250 > GES125 > GES42 = GCtrl；p < 0.05）。形态学数据显示，用染料木黄酮处理后有子宫内膜增殖迹象，尤其是GES125组和GES250组的动物与其他组相比。在GES250组的所有动物中，均观察到子宫鳞状化生迹象。
每日高剂量异黄酮短期治疗可促进去卵巢大鼠子宫内膜鳞状化生。
