Carbonel Adriana Aparecida Ferraz, Lima Patrícia Daniele Azevedo, Lim Jung Jin, Fuchs Luiz Fernando Portugal, Paiotti Ana Paula Ribeiro, Sasso Gisela Rodrigues da Silva, Simões Ricardo Santos, Soares Junior José Maria, Baracat Edmund Chada, Simões Manuel de Jesus
a Department of Gynecology , Paulista School of Medicine/Federal University of São Paulo - EPM/UNIFESP , São Paulo , Brazil.
b Department of Cellular and Molecular Medicine , University of Ottawa , Ottawa , Canada.
Gynecol Endocrinol. 2018 Apr;34(4):314-319. doi: 10.1080/09513590.2017.1393510. Epub 2017 Nov 2.
The objective of this study was to evaluate the action of soy isoflavones and 17 beta estradiol on the extracellular matrix in the uterus and mammary gland of diabetic rats. Sixty adult female rats underwent ovariectomy, then randomized into seven groups of ten animals each: Non-diabetic: GI Sham control animals ovariectomized; and GII control ovariectomized that received propylene glycol vehicle. Diabetic: GIII Sham control diabetic animals ovariectomized; GIV ovariectomized diabetic animals receiving propylene glycol vehicle; GV diabetic ovariectomized animals treated with soy isoflavones (150 mg/kg by gavage); GVI ovariectomized diabetic rats treated with estrogen (17b-estradiol, 10 mg/kg, subcutaneously); GVII diabetic ovariectomized animals treated with soy isoflavones (150 mg/kg by gavage), and with estrogen (17b-estradiol, 10 mg/kg combination therapy). Treatments occurred during 30 consecutive days. After animals euthanasia, a portion of the uterus was immersed in liquid nitrogen for molecular biology analysis, the other portion of uterus and mammary glands were removed and processed for paraffin embedding. Soy isoflavones (GV) and 17b estradiol improved the production of compounds of extracellular matrix, such as small leucine-rich proteoglycans (SLRPs). The combination of both therapies had an additive effect in SLRPs expression. Soy isoflavones contribute to the uterine integrity of SLRPs of diabetic rats.
本研究的目的是评估大豆异黄酮和17β-雌二醇对糖尿病大鼠子宫和乳腺细胞外基质的作用。60只成年雌性大鼠接受卵巢切除术，然后随机分为七组，每组10只动物：非糖尿病组：GI为卵巢切除的假手术对照动物；GII为接受丙二醇载体的卵巢切除对照动物。糖尿病组：GIII为卵巢切除的糖尿病假手术对照动物；GIV为接受丙二醇载体的卵巢切除糖尿病动物；GV为用大豆异黄酮（通过灌胃给予150mg/kg）治疗的卵巢切除糖尿病动物；GVI为用雌激素（17β-雌二醇，10mg/kg，皮下注射）治疗的卵巢切除糖尿病大鼠；GVII为用大豆异黄酮（通过灌胃给予150mg/kg）和雌激素（17β-雌二醇，10mg/kg联合治疗）的卵巢切除糖尿病动物。连续30天进行治疗。动物安乐死后，将一部分子宫浸入液氮中进行分子生物学分析，另一部分子宫和乳腺取出并进行石蜡包埋。大豆异黄酮（GV）和17β-雌二醇改善了细胞外基质成分的产生。两种疗法联合在富含亮氨酸的小分子蛋白聚糖（SLRPs）表达上具有相加作用。大豆异黄酮有助于维持糖尿病大鼠子宫中SLRPs的完整性。
