Virus Laboratory, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, Liaoning 110004, China.
Microbiol Immunol. 2011 Feb;55(2):95-9. doi: 10.1111/j.1348-0421.2010.00294.x.
The functions of some proteins encoded by human cytomegalovirus (HCMV) UL/b' genes have been studied; however, systematic analysis of the transcripts for this region is still insufficient. The results of both rapid amplification of cDNA ends (RACE) and cDNA library screening in this study proved that 3' termini of all transcripts in the UL138-UL145 region were located approximately 20 bp downstream from each potential poly (A) signal, which were at the positions of nucleotides 7184, 9954 and 12848 in the UL/b' sequence of the H strain, respectively. Thus, there were at least two large families of polycistronic transcripts in this gene region. The first family of 3'-coterminal transcripts contained UL139, UL140 and UL141 genes, and the second one consisted of UL142, UL143, UL144 and UL145 genes. The 3'-coterminal characterization further confirmed that multiple uses of polyadenylation signals were commonly used by HCMV to utilize genetic information.
人巨细胞病毒（HCMV）UL/b'基因编码的一些蛋白质的功能已经被研究过；然而，对该区域转录本的系统分析仍然不足。本研究中快速扩增 cDNA 末端（RACE）和 cDNA 文库筛选的结果证明，UL138-UL145 区域中所有转录本的 3'末端都位于每个潜在的多聚（A）信号下游约 20 个碱基处，分别位于 H 株 UL/b'序列的核苷酸 7184、9954 和 12848 处。因此，在这个基因区域至少存在两个大型的多顺反子转录本家族。第一个 3'-端共转录本家族包含 UL139、UL140 和 UL141 基因，第二个家族则由 UL142、UL143、UL144 和 UL145 基因组成。3'-端共转录本的特征进一步证实，多聚腺苷酸化信号的多用途被 HCMV 广泛用于利用遗传信息。
