Graduate School for Computing in Medicine and Life Sciences, University of Luebeck Luebeck, Germany.
Front Neurosci. 2011 Oct 10;5:114. doi: 10.3389/fnins.2011.00114. eCollection 2011.
Minimizing the foreign body response is seen as one critical research strategy for implants especially when designed for immune-privileged organs like the brain. The context of this work is to improve deep brain stimulating devices used in a consistently growing spectrum of psychomotor and psychiatric diseases mainly in form of stiff electrodes. Based on the compliance match hypothesis of biocompatibility we present another step forward using flexible implant materials covered with brain cell-mimicking layers. We covered two types of flexible polyimide films with glandular stem cells derived from pancreatic acini. Using real time-PCR and fluorescent immunocytochemistry we analyzed markers representing various cell types of all three germ layers and stemness. The results demonstrate an unchanged differentiation potential of the polyimide fixated cells as measured by mRNA and protein level. Additionally we developed a fibrinous hydrogel coating to protect them against shear forces upon eventual implantation. By repeating previous analysis and additional metabolism tests for all stages we corroborate the validity of this improvement. Consequently we assume that a stem cell-containing cover may provide a native, fully and actively integrating brain-mimicking interface to the neuropil.
将异物反应最小化被视为植入物的一个关键研究策略，特别是当设计用于免疫特权器官如大脑时。这项工作的背景是改进用于不断增长的精神运动和精神疾病谱的深部脑刺激装置，主要是以僵硬的电极的形式。基于生物相容性的顺应性匹配假说，我们使用覆盖有类似于脑细胞的层的柔性植入材料向前迈进了一步。我们用源自胰腺腺泡的腺干细胞覆盖了两种类型的柔性聚酰亚胺薄膜。使用实时 PCR 和荧光免疫细胞化学，我们分析了代表所有三个胚层和干细胞的各种细胞类型的标志物。结果表明，通过 mRNA 和蛋白质水平测量，聚酰亚胺固定细胞的分化潜力保持不变。此外，我们开发了一种纤维状水凝胶涂层，以在最终植入时保护它们免受剪切力的影响。通过对所有阶段重复以前的分析和额外的代谢测试，我们证实了这种改进的有效性。因此，我们假设含有干细胞的覆盖物可以为神经突提供类似于天然的、完全和主动整合的脑模拟界面。
