Nanosystem Research Institute, National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
J Biomed Mater Res B Appl Biomater. 2011 Apr;97(1):96-104. doi: 10.1002/jbm.b.31790. Epub 2011 Feb 2.
A polymeric material coated with a hydroxyapatite (HA) layer would be useful as a flexible percutaneous device with good biocompatibility and resistance to bacterial infection. We have recently developed a simple, safe, and mild coating process to form an HA layer on the surfaces of polymeric materials. In this study, our coating process was applied to an ethylene-vinyl alcohol copolymer film. The resulting HA-coated film was percutaneously implanted in the scalp of a rat to examine the stability and biocompatibility of the HA layer. From the results of histological analysis, the HA layer remained undissolved on the film surface under the skin tissue even 3 days after implantation. Owing to the good biocompatibility of HA, the HA-coated film suppressed a host's foreign-body response and integrated with the surrounding skin tissue for as long as 14 days, in a similar fashion to a conventional percutaneous device composed of ceramic HA. Immobilization of a cell adhesion protein, laminin, into the HA layer was found to improve the adhesion strength between the film and the surrounding skin tissue without compromising good biocompatibility of HA. Our coating process to form HA and laminin-HA composite layers would be useful in fabricating polymeric percutaneous devices with a reduced risk of bacterial infection, although further in vivo studies are required.
一种涂覆有羟基磷灰石 (HA) 层的聚合材料将作为一种具有良好生物相容性和抗细菌感染能力的柔性经皮装置非常有用。我们最近开发了一种简单、安全、温和的涂覆工艺，可在聚合材料表面形成 HA 层。在这项研究中，我们的涂覆工艺应用于乙烯-乙烯醇共聚物薄膜。将得到的涂覆有 HA 的薄膜经皮植入大鼠头皮，以检查 HA 层的稳定性和生物相容性。从组织学分析的结果来看，即使在植入后 3 天，HA 层仍在皮肤组织下的薄膜表面保持不溶解。由于 HA 的良好生物相容性，HA 涂覆的薄膜抑制了宿主的异物反应，并与周围的皮肤组织整合在一起长达 14 天，与由陶瓷 HA 组成的传统经皮装置相似。发现将细胞粘附蛋白层粘连蛋白固定到 HA 层中可以提高薄膜与周围皮肤组织之间的粘附强度，而不会损害 HA 的良好生物相容性。我们的涂覆工艺形成 HA 和层粘连蛋白-HA 复合层将有助于制造具有降低细菌感染风险的经皮聚合物装置，尽管还需要进一步的体内研究。
