Department of Biology and Institute for Genome Science and Policy Center for Systems Biology, Duke University, Durham, NC 27708, USA.
Dev Cell. 2011 Oct 18;21(4):770-82. doi: 10.1016/j.devcel.2011.09.009.
Stress responses in plants are tightly coordinated with developmental processes, but interaction of these pathways is poorly understood. We used genome-wide assays at high spatiotemporal resolution to understand the processes that link development and stress in the Arabidopsis root. Our meta-analysis finds little evidence for a universal stress response. However, common stress responses appear to exist with many showing cell type specificity. Common stress responses may be mediated by cell identity regulators because mutations in these genes resulted in altered responses to stress. Evidence for a direct role for cell identity regulators came from genome-wide binding profiling of the key regulator SCARECROW, which showed binding to regulatory regions of stress-responsive genes. Coexpression in response to stress was used to identify genes involved in specific developmental processes. These results reveal surprising linkages between stress and development at cellular resolution, and show the power of multiple genome-wide data sets to elucidate biological processes.
植物的应激反应与发育过程密切协调，但这些途径的相互作用还知之甚少。我们使用高时空分辨率的全基因组分析来了解拟南芥根中连接发育和应激的过程。我们的综合分析几乎没有发现普遍应激反应的证据。然而，似乎存在许多具有细胞类型特异性的共同应激反应。共同的应激反应可能是由细胞身份调节剂介导的，因为这些基因的突变导致对应激的反应发生改变。细胞身份调节剂直接作用的证据来自关键调节因子 SCARECROW 的全基因组结合分析，结果显示其与应激反应基因的调控区域结合。对响应应激的共表达的分析，鉴定了参与特定发育过程的基因。这些结果揭示了在细胞分辨率下应激和发育之间令人惊讶的联系，并展示了多个全基因组数据集阐明生物学过程的强大功能。
