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木质纤维素抑制化合物对新分离的耐热东方伊萨酵母生长和乙醇发酵的影响。

Effect of lignocellulosic inhibitory compounds on growth and ethanol fermentation of newly-isolated thermotolerant Issatchenkia orientalis.

机构信息

National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.

出版信息

Bioresour Technol. 2011 Sep;102(17):8099-104. doi: 10.1016/j.biortech.2011.06.035. Epub 2011 Jun 15.

DOI:10.1016/j.biortech.2011.06.035
PMID:21737262
Abstract

A newly isolated thermotolerant ethanologenic yeast strain, Issatchenkia orientalis IPE 100, was able to produce ethanol with a theoretical yield of 85% per g of glucose at 42°C. Ethanol production was inhibited by furfural, hydroxymethylfurfural and vanillin concentrations above 5.56 gL(-1), 7.81 gL(-1), and 3.17 gL(-1), respectively, but the strain was able to produce ethanol from enzymatically hydrolyzed steam-exploded cornstalk with 93.8% of theoretical yield and 0.91 gL(-1)h(-1) of productivity at 42°C. Therefore, I. orientalis IPE 100 is a potential candidate for commercial lignocelluloses-to-ethanol production.

摘要

一株新分离的耐热产乙醇酵母菌株东方伊萨酵母(Issatchenkia orientalis)IPE 100,能够在 42°C 下每克葡萄糖理论产 85%的乙醇。呋喃甲醛、5-羟甲基糠醛和香草醛的浓度超过 5.56、7.81 和 3.17 g/L 时,会抑制乙醇的生成,但该菌株能够从酶解蒸汽爆破玉米秸秆中生产乙醇,在 42°C 时理论产率为 93.8%,生产速率为 0.91 g/L/h。因此,东方伊萨酵母 IPE 100 是木质纤维素生产燃料乙醇的潜在候选菌株。

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