National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
Bioresour Technol. 2011 Sep;102(17):8099-104. doi: 10.1016/j.biortech.2011.06.035. Epub 2011 Jun 15.
A newly isolated thermotolerant ethanologenic yeast strain, Issatchenkia orientalis IPE 100, was able to produce ethanol with a theoretical yield of 85% per g of glucose at 42°C. Ethanol production was inhibited by furfural, hydroxymethylfurfural and vanillin concentrations above 5.56 gL(-1), 7.81 gL(-1), and 3.17 gL(-1), respectively, but the strain was able to produce ethanol from enzymatically hydrolyzed steam-exploded cornstalk with 93.8% of theoretical yield and 0.91 gL(-1)h(-1) of productivity at 42°C. Therefore, I. orientalis IPE 100 is a potential candidate for commercial lignocelluloses-to-ethanol production.
一株新分离的耐热产乙醇酵母菌株东方伊萨酵母(Issatchenkia orientalis)IPE 100,能够在 42°C 下每克葡萄糖理论产 85%的乙醇。呋喃甲醛、5-羟甲基糠醛和香草醛的浓度超过 5.56、7.81 和 3.17 g/L 时,会抑制乙醇的生成,但该菌株能够从酶解蒸汽爆破玉米秸秆中生产乙醇,在 42°C 时理论产率为 93.8%,生产速率为 0.91 g/L/h。因此,东方伊萨酵母 IPE 100 是木质纤维素生产燃料乙醇的潜在候选菌株。