Centre de Recherche et de Formation sur les Plantes Médicinales, Université de Lomé, Togo.
Asian Pac J Trop Med. 2011 Oct;4(10):808-13. doi: 10.1016/S1995-7645(11)60199-5.
To investigate the antioxidant, antimicrobial, antiplasmodial, acute toxicity and haemolytic activities of methanolic extracts of three plants. Phytochemical analysis to determine the phenolic contents was also carried out.
The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging, NCCLS broth microdilution and Plasmodium Lactate Dehydrogenase (pLDH) assays were used to determine antioxidant, antimicrobial and antiplasmodial activities, respectively. Haemolysis assay was conducted on A(+) human red blood cells and acute toxicity on male Swiss albino mice. Phenolics were quantitatively determined using spectrophotometric methods.
The DPPH assay yielded interesting antioxidant activities of methanolic extract of Parinari curatellifolia (P. curatellifolia) and Entada africana (E. africana) (IC(50) were 0.20±0.01 μg/mL and 0.47±0.01 μg/mL, respectively). This activity was highly correlated with phenolic contents of extracts. The antimicrobial tests displayed minimal inhibitory concentrations (MICs) values ranging from 0.90 to 1.80 mg/mL for Serratia marcescens (S. marcescens) the most susceptible bacterial strain. MIC value was 1.20 mg/mL for susceptible fungal strains including Mucor rouxi (M. rouxi), Fusarium oxyporum (F. oxyporum) and Rhizopus nigricans (R. nigricans). pLDH assay showed moderate antiplasmodial activity of Balanites aegyptiaca (B. aegyptiaca) (IC(50) = 24.56±3.45 μg/mL), however this extract was highly haemolytic and toxic in mice (LD(50) = 625±128 mg/kg).
Our results support in part the use of the selected plants in the treatment of microbial infections. In addition the plant showed interesting antioxidant activity that could be useful in the management of oxidative stress.
研究三种植物的甲醇提取物的抗氧化、抗菌、抗疟原虫、急性毒性和溶血活性。还进行了植物化学分析以确定酚类含量。
使用 2,2-二苯基-1-苦基肼（DPPH）自由基清除、NCCLS 肉汤微量稀释和疟原乳酸脱氢酶（pLDH）测定法分别测定抗氧化、抗菌和抗疟原虫活性。溶血试验在 A(+)人红细胞上进行，急性毒性在雄性瑞士白化小鼠上进行。使用分光光度法定量测定酚类。
DPPH 测定法显示 Parinari curatellifolia（P. curatellifolia）和 Entada africana（E. africana）的甲醇提取物具有有趣的抗氧化活性（IC（50）分别为 0.20±0.01 μg/mL 和 0.47±0.01 μg/mL）。这种活性与提取物的酚含量高度相关。抗菌试验显示，最敏感的细菌菌株粘质沙雷氏菌（S. marcescens）的最小抑菌浓度（MIC）值范围为 0.90 至 1.80 mg/mL。对易感性真菌菌株如毛霉（M. rouxi）、尖孢镰刀菌（F. oxyporum）和黑根霉（R. nigricans）的 MIC 值为 1.20 mg/mL。pLDH 测定法显示 Balanites aegyptiaca（B. aegyptiaca）具有中度抗疟原虫活性（IC（50）=24.56±3.45 μg/mL），但该提取物在小鼠中具有高度溶血和毒性（LD（50）=625±128 mg/kg）。
我们的结果部分支持了所选植物在治疗微生物感染中的应用。此外，该植物表现出有趣的抗氧化活性，这在管理氧化应激方面可能很有用。
