Muthusami Sridhar, Ramachandran Ilangovan, Krishnamoorthy Senthilkumar, Govindan Ramajayam, Narasimhan Srinivasan
Department of Endocrinology, Dr.ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus, Chennai, India.
Growth Horm IGF Res. 2011 Dec;21(6):343-8. doi: 10.1016/j.ghir.2011.09.002. Epub 2011 Oct 19.
Osteoporosis is a public health problem which is associated with significant morbidity and mortality. Growth factors are produced locally in the bone and control cellular events such as induction of bone growth. Signaling through the Insulin-like growth factor (IGF)-I receptor (IGF-IR) by locally synthesized IGF - I or IGF-II in osteoblast is considered crucial for normal development and for bone remodeling. Traditional use of Cissus quadrangularis (C. quadrangularis) in the treatment of bone disorders have been documented, however its regulatory effects on IGF system components remain largely unknown. The present study is employed to delineate the effects of ethanolic extract of C. quadrangularis on the regulation of IGF system components in human osteoblast like SaOS-2 cells. RT-PCR analysis revealed an increase in the mRNA expression of IGF-I, IGF-II, IGF-IR in cells treated with C. quadrangularis when compared with control cells. The mRNA expression of IGF binding protein-3 (IGFBP-3) did not differ significantly between control and C. quadrangularis treated cells. Immunoradiometric analysis revealed increased levels of IGF-I, IGF-II and IGFBP-3 in the conditioned medium of C. quadrangularis treated cultures when compared with control. Western blotting analysis revealed increase in protein levels of IGF-IR in cells treated with C. quadrangularis. These results indicate positive regulation of C. quadrangularis on the IGF system components of human osteoblast like SaOS-2 cells.
骨质疏松症是一个与高发病率和死亡率相关的公共卫生问题。生长因子在骨骼局部产生，并控制诸如诱导骨生长等细胞活动。成骨细胞中局部合成的胰岛素样生长因子（IGF）-I或IGF-II通过IGF-I受体（IGF-IR）发出信号，被认为对正常发育和骨重塑至关重要。西番莲（Cissus quadrangularis）传统上用于治疗骨骼疾病已有记载，然而其对IGF系统成分的调节作用仍 largely未知。本研究旨在描述西番莲乙醇提取物对人成骨样SaOS-2细胞中IGF系统成分调节的影响。逆转录聚合酶链反应（RT-PCR）分析显示，与对照细胞相比，用西番莲处理的细胞中IGF-I、IGF-II、IGF-IR的mRNA表达增加。在对照细胞和经西番莲处理的细胞之间，IGF结合蛋白-3（IGFBP-3）的mRNA表达没有显著差异。免疫放射分析显示，与对照相比，经西番莲处理的培养物的条件培养基中IGF-I、IGF-II和IGFBP-3的水平增加。蛋白质印迹分析显示，用西番莲处理的细胞中IGF-IR的蛋白质水平增加。这些结果表明西番莲对人成骨样SaOS-2细胞的IGF系统成分有正向调节作用。
