Noor Zairin, Kania Nia, Setiawan Bambang
Research Center for Osteoporosis, Department of Orthopaedic and Traumatology, Ulin General Hospital, Medical Faculty, Lambung Mangkurat University, Jl. A. Yani Km 2 No.43, Banjarmasin, South Kalimantan Indonesia.
Research Center for Osteoporosis, Department of Pathology, Ulin General Hospital, Medical Faculty, Lambung Mangkurat University, Banjarmasin, South Kalimantan Indonesia.
J Diabetes Metab Disord. 2014 Sep 2;13(1):91. doi: 10.1186/s40200-014-0091-4. eCollection 2014.
The model of bilaterally ovariectomized rats mimics the accelerated bone loss observed in postmenopausal women due to estrogen deficiency. Although calcium is main mineral in bone, previous study in human showed there is hypermineralization and higher calcium level in hydroxyapatite crystal structure from osteoporosis patients. This study was aimed to investigate the effect of time course ovariectomized on tibia bone turn over markers, mineral elements, hydroxyapatite crystale, mesostructure, and histomorphometry.
A total of 30 Wistar female rats were randomly assigned into three groups (n = 10 each): control group, ovariectomy group follow up for one month and two month. All animals procedures was according to Animal Ethics Guidelines and approval by ethic committee of the Medical Faculty, Lambung Mangkurat University which obtained prior the study. Expression of osteocalcin (OC) and C-telopeptyde collagen type I (CTX) was analyzed by ELISA method. Tibia bone mineral element was measured using X-Ray Fluorescence. Hydroxyapatite crystale structure was analyzed using X-Ray Diffracttion. Mesostructure was determined using Scanning Electron Microscope. Histomorphometry was analyzed using BoneJ software analyzer. ANOVA test was used to analyze the different level of serum bone turnover markers and bone mineral elements.
Serum OC and CTX were significantly decrease in one month and two month after ovariectomized groups compared to sham-operated group (P < 0.05). The levels Ca, P, Fe, Cu, Zn, Ni, Ca/P, and Cu/Zn were not significantly different in all groups (P > 0.05). The structure of hydroxyapatite crystal in one month and two month after ovariectomized groups were different compared with sham-operated control group. Mesostructure of tibia bone after one and two month ovariectomized procedure significantly different than that in sham-operated rats. The level of trabecular volume were lower significantly on OVX-1 and OVX-2 groups compared with sham group (P < 0.05). The trabecular thickness and spacing were increase significantly on OVX-1 and OVX-2 groups compared with sham group (P < 0.05). The trabecular number were significantly decrease OVX-1 and OVX-2 groups than that sham group (P < 0.05).
We found that two month after ovariectomized decrease serum osteocalcin but not change bone mineral elements in rats. Also, we found the difference of lattice parameter of hydroxyapatite crystale structure and trabecular properties which determined bone mesostructure.
双侧卵巢切除大鼠模型模拟了绝经后女性因雌激素缺乏而出现的加速骨质流失。尽管钙是骨骼中的主要矿物质，但先前对人类的研究表明，骨质疏松患者的羟基磷灰石晶体结构存在矿化过度和钙水平较高的情况。本研究旨在探讨卵巢切除时间进程对胫骨骨转换标志物、矿物质元素、羟基磷灰石晶体、细观结构和组织形态计量学的影响。
总共30只Wistar雌性大鼠被随机分为三组（每组n = 10）：对照组、卵巢切除术后随访1个月组和2个月组。所有动物实验程序均按照动物伦理准则进行，并获得了兰邦孟库拉特大学医学院伦理委员会的批准，该批准在研究之前获得。采用酶联免疫吸附测定法分析骨钙素（OC）和I型胶原C末端肽（CTX）的表达。使用X射线荧光法测量胫骨骨矿物质元素。使用X射线衍射法分析羟基磷灰石晶体结构。使用扫描电子显微镜确定细观结构。使用BoneJ软件分析仪进行组织形态计量学分析。采用方差分析检验分析血清骨转换标志物和骨矿物质元素的不同水平。
与假手术组相比，卵巢切除术后1个月和2个月组的血清OC和CTX显著降低（P < 0.05）。所有组中钙、磷、铁、铜、锌、镍、钙/磷和铜/锌的水平无显著差异（P > 0.05）。卵巢切除术后1个月和2个月组的羟基磷灰石晶体结构与假手术对照组不同。卵巢切除术后1个月和2个月的胫骨骨细观结构与假手术大鼠显著不同。与假手术组相比，OVX - 1组和OVX - 2组的骨小梁体积显著降低（P < 0.05）。与假手术组相比，OVX - 1组和OVX - 2组的骨小梁厚度和间距显著增加（P < 0.05）。与假手术组相比，OVX - 1组和OVX - 2组的骨小梁数量显著减少（P < 0.05）。
我们发现卵巢切除术后2个月大鼠血清骨钙素降低，但骨矿物质元素未改变。此外，我们发现了羟基磷灰石晶体结构的晶格参数和决定骨细观结构的骨小梁特性的差异。
