Novak S, D'Amore T, Stewart G G
Brewing Research Department, John Labatt Limited, London, Ont., Canada.
FEBS Lett. 1990 Aug 20;269(1):202-4. doi: 10.1016/0014-5793(90)81154-g.
The transport of glucose and maltose in Saccharomyces cerevisiae was observed to occur by both high and low affinity transport systems. A spontaneously isolated 2-deoxy-D-glucose resistant mutant was observed to transport glucose and maltose only by the high affinity transport systems. Associated with this was an increase in the Vmax values, indicating derepression of the high affinity transport systems. The low affinity transport systems could not be detected. This mutant will be important in examining the repression regulatory and sugar transport mechanisms in yeast.
观察到酿酒酵母中葡萄糖和麦芽糖的转运通过高亲和力和低亲和力转运系统进行。观察到一个自发分离的2-脱氧-D-葡萄糖抗性突变体仅通过高亲和力转运系统转运葡萄糖和麦芽糖。与此相关的是Vmax值增加，表明高亲和力转运系统的去阻遏。未检测到低亲和力转运系统。该突变体对于研究酵母中的阻遏调节和糖转运机制将具有重要意义。
