Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Int Microbiol. 2011 Mar;14(1):25-32. doi: 10.2436/20.1501.01.132.
Forty nine Campylobacter jejuni isolates from cattle feces collected from Alberta feedlots and 50 clinical C. jejuni isolates from people in Alberta were tested for the presence of 14 genes encoding putative virulence factors by PCR. These included genes implicated in adherence and colonization (flaC, cadF, docC, racR, jlpA, peb1, and dnaJ), invasion (virB11, ciaB, pldA, and iamA) and protection against harsh conditions (htrA, cbrA, and sodB). The genes examined were widely distributed in both the cattle fecal isolates and the human isolates. Of the isolates tested, 67% contained all of the genes except virB11. The cadF gene was found in 100% of the isolates tested. The presence or absence of virulence-associated genes was not associated with the ability of the organism to colonize birds. All of the C. jejuni isolates used to challenge birds were able to colonize the animals regardless of virulence gene profile. While some diversity in the profile of the occurrence of virulence-associated genes in C. jejuni exists, the distribution of these putative virulence-associated genes isolates from feedlot cattle feces and humans in Alberta was similar. In addition it was not possible to predict the ability of the selected isolates to colonize young chicks based on the presence of these genes coding for virulence determinants.
从艾伯塔省饲养场采集的 49 株牛粪便中的空肠弯曲杆菌分离株和 50 株来自艾伯塔省人的临床空肠弯曲杆菌分离株，通过 PCR 检测了 14 个编码潜在毒力因子的基因的存在。这些基因包括与粘附和定植相关的基因（flaC、cadF、docC、racR、jlpA、peb1 和 dnaJ）、侵袭相关的基因（virB11、ciaB、pldA 和 iamA）和保护免受恶劣条件影响的基因（htrA、cbrA 和 sodB）。在牛粪便分离株和人分离株中广泛分布了所检查的基因。在所测试的分离株中，67%的分离株含有除 virB11 以外的所有基因。cadF 基因存在于 100%的测试分离株中。毒力相关基因的存在与否与该生物体定植鸟类的能力无关。用于挑战鸟类的所有空肠弯曲杆菌分离株都能够定植动物，而与毒力基因谱无关。虽然空肠弯曲杆菌中存在与毒力相关基因发生的多样性，但这些假定的毒力相关基因在艾伯塔省饲养场牛粪便和人类分离株中的分布相似。此外，基于编码毒力决定因素的这些基因的存在，不可能预测所选分离株定植幼雏的能力。
