Center of Safety Evaluation, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
Phytother Res. 2012 May;26(5):716-21. doi: 10.1002/ptr.3633. Epub 2011 Oct 20.
The present study aimed to investigate whether Lycium barbarum polysaccharides (LBP) would protect against doxorubicin (DOX)-induced testicular toxicity. Male Sprague-Dawley rats were treated with distilled water (4 mL/kg) or LBP (200 mg/kg, p.o.) daily for 10 days and followed by saline (0.9 %, 10 mL/kg) or DOX (10 mg/kg) intravenous injection at day 7. Pretreatment with LBP ameliorated DOX-induced reduction in the testicular weights, sperm concentrations and percentage of motile sperms, as well as the increase in abnormal sperm rate. LBP administration to DOX-treated rats successfully reversed the changes in MDA and GHS-Px levels. Compared with the control, pretreatment with LBP significantly increased the plasma testosterone level in the LBP + DOX group. The histopathology examinations further confirmed that LBP effectively attenuated DOX-induced severe degenerative changes of seminiferous tubules. This study illustrated the capability of LBP in attenuating testicular oxidative stress and protecting testis-specific toxicity in DOX-exposed rats.
本研究旨在探讨枸杞多糖（LBP）是否可以预防阿霉素（DOX）诱导的睾丸毒性。雄性 Sprague-Dawley 大鼠每天用蒸馏水（4 mL/kg）或 LBP（200 mg/kg，po）处理 10 天，然后在第 7 天用生理盐水（0.9%，10 mL/kg）或 DOX（10 mg/kg）静脉注射。LBP 预处理可改善 DOX 引起的睾丸重量、精子浓度和精子活动率的降低，以及精子畸形率的增加。LBP 给药可使 DOX 处理大鼠 MDA 和 GHS-Px 水平的变化得到逆转。与对照组相比，LBP 预处理可使 LBP+DOX 组的血浆睾酮水平显著升高。组织病理学检查进一步证实，LBP 可有效减轻 DOX 诱导的生精小管严重退行性改变。本研究表明，LBP 具有减轻睾丸氧化应激和保护 DOX 暴露大鼠睾丸特异性毒性的能力。
