Department of Histology and Embryology, Clinical Anatomy & Reproductive Medicine Application Institute, University of South China, Hengyang, China.
School of Public and Health, Guilin Medical University, Guilin, China.
Front Endocrinol (Lausanne). 2020 Apr 17;11:164. doi: 10.3389/fendo.2020.00164. eCollection 2020.
The objective of this study was to investigate the protective effects of polysaccharides (LBP) on testicular spermatogenic function in streptozotocin (STZ)-induced diabetic rats. Compared to the control group, blood glucose levels were significantly increased and the insulin resistance was markedly aggravated in STZ-induced diabetic rats. Further, the weight of testis and epididymis and the sperm number and motility were decreased in diabetic rats. Pathological changes were also observed in the spermatogenic tubules, along with a decreased number of spermatogenic cells, downregulated proliferating cell nuclear antigen (PCNA) expression, and increased cell apoptosis in the testes. Compared to the saline-treated diabetic rat group, metformin and LBP treatment significantly decreased the level of blood glucose and improved insulin resistance and testicular function. After treatment with metformin and LBP, the pathological changes in the spermatogenic tubules improved significantly, with an increase in the number of spermatogenic cells, upregulation of PCNA, and suppression of apoptosis in the testes. The expressions of sirtuin 1 (SIRT1) and hypoxia-inducible factor 1-alpha (HIF-1α) in diabetic testes were also upregulated by metformin or LBP treatment. In summary, LBP exerted protective effects by increasing cell proliferation, inhibiting cell apoptosis, and regulating SIRT1/HIF-1α expression in the testes of diabetic rats.
本研究旨在探讨多糖（LBP）对链脲佐菌素（STZ）诱导的糖尿病大鼠睾丸生精功能的保护作用。与对照组相比，STZ 诱导的糖尿病大鼠血糖水平显著升高，胰岛素抵抗明显加重。此外，糖尿病大鼠的睾丸和附睾重量、精子数量和活力下降。生精小管也出现了病理变化，精原细胞数量减少，增殖细胞核抗原（PCNA）表达下调，睾丸细胞凋亡增加。与生理盐水处理的糖尿病大鼠组相比，二甲双胍和 LBP 治疗显著降低了血糖水平，改善了胰岛素抵抗和睾丸功能。用二甲双胍和 LBP 治疗后，生精小管的病理变化明显改善，精原细胞数量增加，PCNA 上调，睾丸细胞凋亡受到抑制。糖尿病大鼠睾丸中 SIRT1（SIRT1）和缺氧诱导因子 1-α（HIF-1α）的表达也被二甲双胍或 LBP 治疗上调。总之，LBP 通过增加细胞增殖、抑制细胞凋亡和调节 SIRT1/HIF-1α 表达来发挥对糖尿病大鼠睾丸的保护作用。
