Chen Lian, Xu Haigen, Li Hong, Wu Jun, Ding Hui, Liu Yan
Nanjing Institute of Environmental Sciences under Ministry of Environmental Protection, 8 Jiangwangmiao Street, Nanjing 210042, Jiangsu, China; E-Mails:
Int J Mol Sci. 2011;12(9):5993-8. doi: 10.3390/ijms12095993. Epub 2011 Sep 16.
We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of "100 of the world's worst invasive alien species". The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) method was used to isolate microsatellite loci, and polymorphism was explored with 29 individuals collected in an invasive region from China. These primers showed a number of alleles per locus ranging from three to 13. The ranges of observed and expected heterozygosity were 0.310-0.966 and 0.523-0.898, respectively. These microsatellite markers described here will be useful for population genetic studies of P. canaliculata.
我们报告了对福寿螺(Pomacea canaliculata)16个多态微卫星标记的特征分析,福寿螺是列入“世界100种最严重入侵外来物种”名单的一种害虫。采用AFLP(扩增片段长度多态性)快速分离含重复序列(FIASCO)方法分离微卫星位点,并利用从中国一个入侵地区采集的29个个体探究其多态性。这些引物每个位点显示的等位基因数为3至13个。观察到的杂合度范围和预期杂合度范围分别为0.310 - 0.966和0.523 - 0.898。这里描述的这些微卫星标记将有助于福寿螺的群体遗传学研究。