Laboratory of Immunosenescence, Institute of Biomedical Research, Faculty of Biosciences, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
J Clin Immunol. 2013 May;33(4):876-9. doi: 10.1007/s10875-012-9857-y. Epub 2013 Jan 29.
CD4(+)CD25(+)Foxp3(+) regulatory T (Treg) cell dysfunction has been documented in various autoimmune disorders, but not in antiphospholipid syndrome (APS) so far.
In this cross-sectional study, we aim to investigate CD4(+)CD25(+)Foxp3(+) Treg cells, CD3(+)CD19(-) T cells and CD3(-)CD19(+) B cells in patients with primary APS and healthy controls. Cell subtypes were immunophenotyped using specific monoclonal antibodies (anti-CD3 CY5, anti-CD4 FITC, anti-CD25, anti-Foxp3, anti-CD19 PE) and flow cytometry.
Twenty patients with APS and 20 age- and sex-matched controls were studied. The percentage of total lymphocytes, activated Th cells (CD4+CD25+), Treg cells and CD3(-)CD19(+) B cells were found significantly lower in APS patients as compared to controls (all p < 0.05).
A dysfunction in CD4(+)CD25(+)Foxp3(+) Treg cells may represent one of the mechanisms leading to autoimmunity in APS patients. The decreased number of CD3(-)CD19(+) B cells of APS patients warrants further elucidation.
在各种自身免疫性疾病中已经发现 CD4(+)CD25(+)Foxp3(+)调节性 T（Treg）细胞功能障碍，但迄今为止在抗磷脂综合征（APS）中尚未发现。
在这项横断面研究中，我们旨在研究原发性 APS 患者和健康对照者中的 CD4(+)CD25(+)Foxp3(+)Treg 细胞、CD3(+)CD19(-)T 细胞和 CD3(-)CD19(+)B 细胞。使用特异性单克隆抗体（抗-CD3 CY5、抗-CD4 FITC、抗-CD25、抗-Foxp3、抗-CD19 PE）和流式细胞术对细胞亚型进行免疫表型分析。
研究了 20 例 APS 患者和 20 名年龄和性别匹配的对照者。与对照组相比，APS 患者的总淋巴细胞、活化 Th 细胞（CD4+CD25+）、Treg 细胞和 CD3(-)CD19(+)B 细胞的比例明显降低（均 p<0.05）。
CD4(+)CD25(+)Foxp3(+)Treg 细胞功能障碍可能是 APS 患者发生自身免疫的机制之一。APS 患者 CD3(-)CD19(+)B 细胞数量减少值得进一步阐明。
