原发性抗磷脂综合征患者循环中 CD4+CD25+Foxp3+调节性 T 细胞水平降低。
Decreased levels of circulating CD4+CD25+Foxp3+ regulatory T cells in patients with primary antiphospholipid syndrome.
机构信息
Laboratory of Immunosenescence, Institute of Biomedical Research, Faculty of Biosciences, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
出版信息
J Clin Immunol. 2013 May;33(4):876-9. doi: 10.1007/s10875-012-9857-y. Epub 2013 Jan 29.
INTRODUCTION
CD4(+)CD25(+)Foxp3(+) regulatory T (Treg) cell dysfunction has been documented in various autoimmune disorders, but not in antiphospholipid syndrome (APS) so far.
METHODS
In this cross-sectional study, we aim to investigate CD4(+)CD25(+)Foxp3(+) Treg cells, CD3(+)CD19(-) T cells and CD3(-)CD19(+) B cells in patients with primary APS and healthy controls. Cell subtypes were immunophenotyped using specific monoclonal antibodies (anti-CD3 CY5, anti-CD4 FITC, anti-CD25, anti-Foxp3, anti-CD19 PE) and flow cytometry.
RESULTS
Twenty patients with APS and 20 age- and sex-matched controls were studied. The percentage of total lymphocytes, activated Th cells (CD4+CD25+), Treg cells and CD3(-)CD19(+) B cells were found significantly lower in APS patients as compared to controls (all p < 0.05).
CONCLUSION
A dysfunction in CD4(+)CD25(+)Foxp3(+) Treg cells may represent one of the mechanisms leading to autoimmunity in APS patients. The decreased number of CD3(-)CD19(+) B cells of APS patients warrants further elucidation.
简介
在各种自身免疫性疾病中已经发现 CD4(+)CD25(+)Foxp3(+)调节性 T（Treg）细胞功能障碍，但迄今为止在抗磷脂综合征（APS）中尚未发现。
方法
在这项横断面研究中，我们旨在研究原发性 APS 患者和健康对照者中的 CD4(+)CD25(+)Foxp3(+)Treg 细胞、CD3(+)CD19(-)T 细胞和 CD3(-)CD19(+)B 细胞。使用特异性单克隆抗体（抗-CD3 CY5、抗-CD4 FITC、抗-CD25、抗-Foxp3、抗-CD19 PE）和流式细胞术对细胞亚型进行免疫表型分析。
结果
研究了 20 例 APS 患者和 20 名年龄和性别匹配的对照者。与对照组相比，APS 患者的总淋巴细胞、活化 Th 细胞（CD4+CD25+）、Treg 细胞和 CD3(-)CD19(+)B 细胞的比例明显降低（均 p<0.05）。
结论
CD4(+)CD25(+)Foxp3(+)Treg 细胞功能障碍可能是 APS 患者发生自身免疫的机制之一。APS 患者 CD3(-)CD19(+)B 细胞数量减少值得进一步阐明。
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