利用SRAP标记对岷江百合自然居群进行遗传多样性分析

[Genetic diversity analysis of natural groups of Lilium sargentiae by SRAP markers].

作者信息

Zhi Li, Teng Zhonghua, Liu Xu, Li Mingyang

机构信息

College of Horticulture and Landscape Architecture, Southwest University, Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1921-6.

PMID:22016960

Abstract

Genetic diversity of Lilium sargentiae was detected in this paper by sequence-related amplified polymorphism (SRAP) marker. One hundred wild samples were collected from 10 places, and 15 SRAP primer combinations were used for determination. NTSYS-pc2.1 and POPGEN1.32 were used for data analysis. The results showed that a total of 170 clear DNA bands were amplified, 163 of which were polymorphic. The proportion of polymorphic loci was 90.58% on the level of species. Nei's (1973) gene diversity (H) was 0.2631, Shannon's Information index was 0.3661, the G(st), was 0.3672, and the genetic distance ranged from 0.2021 to 0.5749. All materials could be clustered into four groups by UPGMA. The results demonstrated that the genetic diversity of L. sargentiae was rich on the level of species, and the genetic diversity within populations exceeded among populations. The correlations of genetic diversity and distribution were significant in L. sargentiae.

摘要

本文采用序列相关扩增多态性（SRAP）标记对岷江百合的遗传多样性进行了检测。从10个地点采集了100份野生样本，并使用15对SRAP引物组合进行测定。采用NTSYS-pc2.1和POPGEN1.32软件进行数据分析。结果表明，共扩增出170条清晰的DNA条带，其中163条具有多态性。在物种水平上，多态位点比例为90.58%。Nei（1973）基因多样性（H）为0.2631，Shannon信息指数为0.3661，G（st）为0.3672，遗传距离在0.2021至0.5749之间。所有材料通过非加权组平均法（UPGMA）可聚类为4组。结果表明，岷江百合在物种水平上遗传多样性丰富，种群内的遗传多样性超过了种群间的遗传多样性。岷江百合的遗传多样性与分布具有显著相关性。