Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, Spain.
J Agric Food Chem. 2011 Dec 14;59(23):12598-604. doi: 10.1021/jf203965j. Epub 2011 Nov 4.
Polyphenolics are important secondary metabolites in strawberry as they fulfill a wide variety of physiological functions and are beneficial to human health. Seventeen structurally well-defined phenolic compounds including phenylpropanoids, flavonols, flavan-3-ols, and anthocyanins were individually analyzed by LC-MS in the ripe fruits of two cultivars of the commercial strawberry (Fragaria × ananassa Duch., Rosaceae) as well as in accessions of F. vesca, F. moschata, and F. chiloensis. Metabolic analysis revealed that the majority of the compounds analyzed accumulated in a genotype-dependent manner. Transcriptional studies of genes encoding for enzymes of the biosynthetic pathway such as phenylalanine ammonia-lyase, cinnamic acid 4-hydroxylase, chalcone synthase, and flavonoid 3'-hydroxylase could partially explain the different levels of polyphenolics observed in the Fragaria species. The results can provide a sound basis for selecting markers for the development of cultivars with high phenolic content, which can be of value for the food industry.
多酚是草莓中的重要次生代谢物，因为它们具有多种生理功能，对人体健康有益。通过 LC-MS 对两个商业草莓品种（蔷薇科草莓属）以及 F. vesca、F. moschata 和 F. chiloensis 的品种进行了分析，共鉴定出 17 种结构明确的酚类化合物，包括苯丙烷类、黄酮醇、黄烷-3-醇和花青素。代谢分析表明，大多数分析的化合物以基因型依赖的方式积累。对生物合成途径中酶（如苯丙氨酸解氨酶、肉桂酸 4-羟化酶、查尔酮合酶和类黄酮 3'-羟化酶）编码基因的转录研究可以部分解释在草莓属中观察到的多酚水平的不同。这些结果可以为选择高酚含量品种的开发提供可靠的依据，这对食品工业具有重要价值。
