Theodore J G, Kaliraj P
Center for Biotechnology, Anna University, Madras, India.
J Helminthol. 1990 Jun;64(2):105-14. doi: 10.1017/s0022149x00012001.
The surface antigens of the bovine filarial parasite Setaria digitata were isolated by EDTA extraction and purified by affinity chromatography using sepharose bound human filarial (Wuchereria bancrofti) antibodies obtained from chronic human filarial sera. The purified and crude antigens were used in enzyme-linked immunosorbent assay (ELISA) for the detection of serum antibodies in bancroftian filariasis. The purified antigen showed sensitive and specific reactions in ELISA for the detection of antibodies in filarial sera and showed least cross reactivity with other parasitic infections. The crude and purified antigens showed about 18 and 6 peptide bands respectively in SDS-PAGE and about 11 and 6 antigenic bands respectively in enzyme-linked immunoelectrotransfer blot (EITB). The purified antigen was observed to be glycoprotein in nature. It was possible to identify the stage-specific infection in human filariasis by using the crude and purified antigens in EITB.
牛丝状寄生虫指状腹腔丝虫的表面抗原通过乙二胺四乙酸（EDTA）提取法分离，并使用从慢性人类丝虫病血清中获得的、结合在琼脂糖上的人类丝状（班氏吴策线虫）抗体，通过亲和层析法进行纯化。纯化抗原和粗抗原用于酶联免疫吸附测定（ELISA），以检测班氏丝虫病患者血清中的抗体。纯化抗原在ELISA中对丝状血清抗体检测显示出灵敏且特异的反应，与其他寄生虫感染的交叉反应最小。粗抗原和纯化抗原在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）中分别显示约18条和6条肽带，在酶联免疫电转移印迹（EITB）中分别显示约11条和6条抗原带。观察到纯化抗原本质上是糖蛋白。通过在EITB中使用粗抗原和纯化抗原，有可能识别人类丝虫病中的阶段特异性感染。
