Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
DNA Cell Biol. 2012 Apr;31(4):560-7. doi: 10.1089/dna.2011.1332. Epub 2011 Oct 21.
Association between the NAD(P)H: quinine oxidoreductase 1 (NQO1) gene 609 C>T polymorphism and esophageal cancer (EC) has been widely evaluated; however, the results are often irreproducible. We thus aimed to comprehensively evaluate this association through a meta-analysis. Data were extracted from 10 study populations involving 1390 EC patients and 1812 controls, and were analyzed using STATA software. Random-effects model was applied irrespective of between-study heterogeneity, which was assessed by the inconsistency index (I(2)) statistic. Publication bias was weighted by the funnel plot and Egger's test. Genotype distributions of the NQO1 gene 609 C>T polymorphism met Hardy-Weinberg equilibrium in controls for all studies. Allelic comparison indicated that NQO1 609 T allele conferred an increased risk (odds ratio [OR]=1.23; 95% confidence interval [CI]: 1.02-1.49; p=0.035), accompanying significant heterogeneity (I(2)=63.4%, p=0.003) and no publication bias (p(Egger)=0.391). This association was potentially enhanced in homozygous comparison (OR=1.58; 95% CI: 1.03-2.41; p=0.035; I(2)= 55.4%, p(heterogeneity)=0.017 and p(Egger)=0.461). Under dominant and recessive models, similar associations were obtained with an increased, although marginally significant risk. Subgroup analysis by ethnicity supported the risk profiles of the NQO1 gene 609 T allele and 609 TT genotype with EC in Eastern Asians, not in Europeans. Meta-regression analysis indicated that association between the NQO1 gene 609 C>T polymorphism and EC risk was significantly decreased with aging in case-patients (R(2)=-0.57; p=0.042). We expand previous studies by showing that the NQO1 gene 609 C>T polymorphism might contribute to EC occurrence, especially in Eastern Asians.
NAD(P)H：醌氧化还原酶 1（NQO1）基因 609C>T 多态性与食管癌（EC）的关系已被广泛评估；然而，结果往往不可重复。因此，我们旨在通过荟萃分析全面评估这种相关性。从 10 个研究人群中提取了 1390 名 EC 患者和 1812 名对照的数据，并使用 STATA 软件进行分析。无论研究之间是否存在异质性，均采用随机效应模型进行分析，通过不一致性指数（I(2)）统计量评估异质性。通过漏斗图和 Egger 检验评估发表偏倚的权重。所有研究的对照中，NQO1 基因 609C>T 多态性的基因型分布均符合 Hardy-Weinberg 平衡。等位基因比较表明，NQO1609T 等位基因赋予了更高的风险（比值比[OR]=1.23；95%置信区间[CI]：1.02-1.49；p=0.035），并伴有显著的异质性（I(2)=63.4%，p=0.003）和无发表偏倚（p(Egger)=0.391）。在纯合子比较中，这种相关性可能会增强（OR=1.58；95%CI：1.03-2.41；p=0.035；I(2)=55.4%，p(异质性)=0.017 和 p(Egger)=0.461）。在显性和隐性模型下，也得到了相似的关联，风险略有增加。按种族进行的亚组分析支持东亚人群中 NQO1 基因 609T 等位基因和 609TT 基因型与 EC 的风险特征，而在欧洲人群中则不支持。Meta 回归分析表明，病例患者中 NQO1 基因 609C>T 多态性与 EC 风险之间的关联随着年龄的增长而显著降低（R(2)=-0.57；p=0.042）。我们通过显示 NQO1 基因 609C>T 多态性可能导致 EC 的发生，特别是在东亚人群中，扩展了以前的研究。
