Kuga Milton Carlos, Gouveia-Jorge Érica, Tanomaru-Filho Mário, Guerreiro-Tanomaru Juliane Maria, Bonetti-Filho Idomeo, Faria Gisele
Araraquara Dental School, São Paulo State University, Araraquara, SP, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Dec;112(6):e155-9. doi: 10.1016/j.tripleo.2011.05.040. Epub 2011 Oct 21.
The objective of this study was to evaluate the penetration of 2.5% NaOCl associated with 17.0% EDTA, 1.0% citric acid, and 1.0% peracetic acid into dentin tubules.
The roots of 44 bovine incisors were cross-sectioned and 5-mm-long fragments were produced from their middle thirds. The specimens were instrumented with ProTaper hand files, stained in crystal violet, then sectioned mesiodistally. The buccal fragments were divided into 4 groups (n = 9) and subjected to 2 consecutive 10-minute immersion periods in one of the following acid solutions combined with 2.5% NaOCl: 17.0% EDTA (group 1), 1.0% citric acid (group 2), and 1.0% peracetic acid (group 3). Nine fragments were immersed in 2.5% NaOCl (group 4). The analysis of the penetration of NaOCl solutions into dentin was performed by measuring the depth of crystal violet stain that was bleached using a steromicroscope under ×50 magnification. Statistical comparisons were carried out by Kruskal-Wallis and Dunn's tests at the 5% significance level.
Group 1 showed less penetration into dentin than group 4 (P < .05). No statistically significant differences were observed among groups 2, 3, and 4 (P > .05).
Association of NaOCl with acid solutions did not increase its penetration depth into root dentin.
本研究的目的是评估2.5%次氯酸钠与17.0%乙二胺四乙酸、1.0%柠檬酸和1.0%过氧乙酸联合使用时渗入牙本质小管的情况。
将44颗牛切牙的牙根进行横断面切割，从牙根中部三分之一处制取5毫米长的片段。用ProTaper手动锉对标本进行预备，用结晶紫染色，然后进行近远中向切片。将颊侧片段分为4组（每组n = 9），并在以下一种酸溶液与2.5%次氯酸钠的混合溶液中连续浸泡2个10分钟：17.0%乙二胺四乙酸（第1组）、1.0%柠檬酸（第2组）和1.0%过氧乙酸（第3组）。将9个片段浸泡在2.5%次氯酸钠中（第4组）。通过使用体视显微镜在50倍放大倍数下测量被漂白的结晶紫染色深度，来分析次氯酸钠溶液渗入牙本质的情况。在5%的显著性水平下，采用Kruskal-Wallis检验和Dunn检验进行统计学比较。
第1组渗入牙本质的程度低于第4组（P <.05）。在第2、3和4组之间未观察到统计学上的显著差异（P >.05）。
次氯酸钠与酸溶液联合使用并未增加其渗入牙根牙本质的深度。
