Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain.
J Pharm Biomed Anal. 2012 Jan 25;58:34-41. doi: 10.1016/j.jpba.2011.09.027. Epub 2011 Oct 1.
The phenolic fraction of a commercial cranberry syrup, which is purported to have good properties for the prevention of urinary diseases, has been thoroughly characterized using HPLC-DAD-TOF-MS. A study of its antibacterial activity has also been carried out. For this purpose a new HPLC-DAD-TOF-MS method using negative and positive ionization modes was developed and it was thus possible to identify 34 different compounds, nine of which have been tentatively characterized for the first time in cranberry syrup. It is also important to highlight that different coumarins in this matrix were also determined, which, to our knowledge, have not been found previously in the cranberry. The phenolic fraction obtained by HPLC-DAD was found to be 5.47 mg/mL. Catechin and procyanidins belonging to flavanols were the family of compounds found at the highest concentrations (2.37 mg/mL); flavonols were at a concentration of 1.91 mg/mL and phenolic-acid derivatives were found at the lowest concentration (0.15 mg/mL). With regard to antibacterial activity, the incubation of Escherichia coli with cranberry syrup was found to reduce surface hydrophobicity as a function of the concentration of the extract.
采用 HPLC-DAD-TOF-MS 对一种据称具有良好预防尿路疾病特性的商业蔓越莓糖浆的酚类部分进行了全面表征。还对其抗菌活性进行了研究。为此，开发了一种新的 HPLC-DAD-TOF-MS 方法，使用正负离子模式，从而能够鉴定出 34 种不同的化合物，其中有 9 种化合物首次在蔓越莓糖浆中被暂定鉴定。同样重要的是要强调的是，还确定了这种基质中的不同香豆素，据我们所知，这些香豆素以前在蔓越莓中没有发现过。通过 HPLC-DAD 获得的酚类部分的浓度为 5.47mg/mL。属于黄烷醇的儿茶素和原花青素是浓度最高的化合物（2.37mg/mL）；类黄酮的浓度为 1.91mg/mL，而酚酸衍生物的浓度最低（0.15mg/mL）。关于抗菌活性，随着提取物浓度的增加，发现大肠杆菌与蔓越莓糖浆孵育会降低表面疏水性。
