Department of Pathology and Laboratory Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Pathol Res Pract. 2011 Nov 15;207(11):686-90. doi: 10.1016/j.prp.2011.08.009. Epub 2011 Oct 22.
Although thyroid transcription factor-1 (TTF-1) immunoreactivity is considered as a specific marker of lung and thyroid neoplasms, it may be positive in a proportion of extrapulmonary adenocarcinomas. This study examined the expression of TTF-1 in 555 colorectal adenocarcinomas using three commercial monoclonal antibodies: clone SPT24 (Novocastra) and 8G7G3/1 (Dako and Cell Marque), and compared the TTF-1 staining with other immunohistochemical markers, cytokeratin (CK) 7, CK 20, caudal-type homeobox transcription factor 2 (CDX2), and MUC2. The clinicopathological prognostic factors were compared with the TTF-1 expression status. Nuclear TTF-1 staining was detected in 24 cases (4.3%) with the SPT24 antibody and 18 cases (3.2%) with the 8G7G3/1 antibody. All cases positive for 8G7G3/1 were also positive for SPT24. CDX2 was expressed constantly in all 24 TTF-1-positive colorectal cancers, whereas CK7, CK20, and MUC2 were detected in 2 (8.3%), 23 (95.8%), and 8 (33.3%) cases, respectively. There was no correlation between TTF-1 expression and clinicopathological significance. To avoid potential pitfalls, TTF-1 should be interpreted in conjunction with the clinical setting, histological features, and the results of other markers, such as CK7, CK20, and CDX2. MUC2 can be used as supplementary information to confirm difficult cases.
虽然甲状腺转录因子-1（TTF-1）免疫反应被认为是肺和甲状腺肿瘤的特异性标志物，但它可能在一部分肺外腺癌中呈阳性。本研究使用三种商业单克隆抗体：克隆 SPT24（诺华）和 8G7G3/1（Dako 和 Cell Marque），检测了 555 例结直肠腺癌中 TTF-1 的表达，并将 TTF-1 染色与其他免疫组织化学标志物、细胞角蛋白（CK）7、CK 20、尾型同源盒转录因子 2（CDX2）和 MUC2 进行了比较。比较了临床病理预后因素与 TTF-1 表达状态的关系。用 SPT24 抗体检测到 24 例（4.3%）和 8G7G3/1 抗体检测到 18 例（3.2%）细胞核 TTF-1 染色。所有 8G7G3/1 阳性的病例均与 SPT24 阳性。所有 24 例 TTF-1 阳性的结直肠癌均持续表达 CDX2，而 CK7、CK20 和 MUC2 分别在 2（8.3%）、23（95.8%）和 8（33.3%）例中检测到。TTF-1 表达与临床病理意义无相关性。为避免潜在的陷阱，应结合临床背景、组织学特征和其他标志物（如 CK7、CK20 和 CDX2）的结果来解释 TTF-1。MUC2 可作为辅助信息，以确认困难病例。
