红景天苷通过减轻炎症反应和增强人牙周膜干细胞的成骨分化来改善牙周炎。

Salidroside Improves Periodontitis by Mitigating Inflammatory Reactions and Enhancing Osteogenic Differentiation of Human Periodontal Ligament Stem Cells.

作者信息

Li Wanheng, Liu Qing, Chen Siyu, Zhi Mengfan, Yang Peng, Zhang Yuxing, Wu Zhaoyan, Zhang Jun, Feng Qiang

机构信息

Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, People's Republic of China.

Shandong Key Laboratory of Oral Tissue Regeneration, Jinan, People's Republic of China.

出版信息

Drug Des Devel Ther. 2025 Sep 10;19:8097-8114. doi: 10.2147/DDDT.S530311. eCollection 2025.

DOI:10.2147/DDDT.S530311

PMID:40955307

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12433638/

Abstract

PURPOSE

Salidroside (Sal), a significant bioactive compound found in , is documented to possess various pharmacological properties. This study investigated the effects of Sal in alleviating periodontitis.

METHODS

The rat periodontitis model was utilized to assess the therapeutic impact of Sal on periodontitis. Human periodontal ligament stem cells (hPDLSCs) were used to investigate the effect of Sal on lipopolysaccharide (LPS)-inhibited osteogenic differentiation. RNA sequencing (RNA-seq), and Western blot were employed to analyze the genes and proteins impacted by Sal treatment.

RESULTS

Sal significantly alleviated the alveolar bone loss and gingival inflammation in rats periodontitis model. Sal demonstrated a dose-dependent pattern of promoting osteogenesis on hPDLSCs. A concentration of 0.5 μM Sal could effectively counteract the impact of LPS on osteogenic differentiation. Mechanically, Sal inhibited the ratios of phospho-IκBα(p-IκBα)/IκBα and phospho-p65(p-p65)/p65 in Nuclear Factor kappa-B (NF-κB) pathway and reduced the expressions of interleukin-6 (IL-6) and interleukin-8 (IL-8). Sal increased the expression of lymphoid enhancer-binding factor 1 (LEF1).

CONCLUSION

Sal promoted the osteogenic differentiation by inhibiting the activation of the NF-κB pathway and increasing the expression of LEF1.

摘要

目的

红景天苷（Sal）是中发现的一种重要生物活性化合物，已被证明具有多种药理特性。本研究调查了红景天苷在减轻牙周炎方面的作用。

方法

利用大鼠牙周炎模型评估红景天苷对牙周炎的治疗效果。使用人牙周膜干细胞（hPDLSCs）研究红景天苷对脂多糖（LPS）抑制的成骨分化的影响。采用RNA测序（RNA-seq）和蛋白质免疫印迹法分析红景天苷处理后受影响的基因和蛋白质。

结果

红景天苷显著减轻了大鼠牙周炎模型中的牙槽骨丢失和牙龈炎症。红景天苷对hPDLSCs的成骨作用呈剂量依赖性。0.5 μM的红景天苷浓度可有效抵消LPS对成骨分化的影响。机制上，红景天苷抑制核因子κB（NF-κB）通路中磷酸化IκBα（p-IκBα）/IκBα和磷酸化p65（p-p65）/p65的比例，并降低白细胞介素-6（IL-6）和白细胞介素-8（IL-8）的表达。红景天苷增加了淋巴样增强因子1（LEF1）的表达。