Department of Animal Medicine and Surgery, University of Murcia, 30100 Espinardo, Murcia, Spain.
Res Vet Sci. 2012 Oct;93(2):682-7. doi: 10.1016/j.rvsc.2011.09.018. Epub 2011 Oct 22.
The objectives of this study were to perform the optimization and validation of three commercially available immunoassays for the measurement of IgA, IgG, and IgM (Igs) in porcine saliva samples and to determinate if their concentrations may be used to distinguish healthy from diseased animals. Intra and inter assay coefficients of variation were lower than 15% in all cases. All methods showed good linearity and recovery; and detection limits were low enough to detect Igs levels in healthy and diseased animals. The clinical validation showed an increase statistically significant (P<0.05) in the group of diseased animals versus healthy pigs. Therefore, these assays may be used in porcine saliva samples, in addition, the measurement of Igs in saliva could be a practical tool, simple and minimally invasive, to evaluate the humoral immune status of pigs.
本研究的目的是优化和验证三种市售的用于检测猪唾液中 IgA、IgG 和 IgM（Ig）的免疫分析方法，并确定其浓度是否可用于区分健康动物和患病动物。在所有情况下，内和间分析变异系数均低于 15%。所有方法均显示出良好的线性和回收率；检测限足够低，可以检测健康和患病动物的 Ig 水平。临床验证显示，患病动物组与健康猪相比，Ig 水平有统计学意义的增加（P<0.05）。因此，这些检测方法可用于猪唾液样本，此外，唾液中 Ig 的测量可能是一种实用的工具，简单且微创，可用于评估猪的体液免疫状态。
