Department of Physiology and Immunology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
Stem Cells Dev. 2012 Mar 1;21(4):507-12. doi: 10.1089/scd.2011.0491. Epub 2011 Dec 2.
Adult bone marrow mesenchymal stem cells (BMSCs) can be differentiated in vitro to become adipocyte-like cells with lipid vacuoles, similar to adipocytes derived from adult adipose tissue. Little is known regarding the composition of free fatty acids (FFAs) of the in vitro-differentiated adipocytes, or whether it resembles that of native adult adipocytes. We used gas chromatography-mass spectrometry to identify FFA species in BMSC-derived adipocytes and compared them with FFAs found in adipocytes derived from adult adipose tissue. We found that adult adipocytes contained significant percentages of saturated and monounsaturated FFAs, including palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (C18:1); some polyunsaturated FFAs, such as linoleic acid (C18:2), a small percentage of arachidonic acid (C20:4), and very little linolenic acid (C18:3). In comparison, 80%-90% confluent BMSCs contained comparable percentages of palmitic and oleic acids, significantly more arachidonic and stearic acids, very little linoleic acid, and no linolenic acid. After differentiation, compared with adult adipocytes, BMSC-derived adipocytes contained a comparable percentage of palmitic acid, more stearic and arachidonic acids, less oleic acid, almost no linoleic acid, and no detectable linolenic acid. This composition was quite similar to that of undifferentiated BMSCs. The differentiation medium contained only palmitic and stearic acids, with traces of oleic acid; it did not contain the essential polyunsaturated fatty acids. Thus, the composition of FFAs in BMSC-derived adipocytes was altered compared with adult adipocytes. BMSC-derived adipocytes had an altered composition of saturated and monounsaturated FFAs and lacked essential FFAs that may directly affect signaling related to their lipolysis/lipogenesis functions.
成体骨髓间充质干细胞（BMSCs）在体外可分化为具有脂滴的脂肪样细胞，类似于来源于成体脂肪组织的脂肪细胞。关于体外分化的脂肪细胞的游离脂肪酸（FFA）组成，或者其是否类似于天然成体脂肪细胞的组成，目前知之甚少。我们使用气相色谱-质谱联用技术鉴定了来源于 BMSC 的脂肪细胞中的 FFA 种类，并将其与来源于成体脂肪组织的脂肪细胞中的 FFA 进行了比较。我们发现成体脂肪细胞含有大量的饱和和单不饱和 FFA，包括棕榈酸（C16:0）、硬脂酸（C18:0）和油酸（C18:1）；一些多不饱和 FFA，如亚油酸（C18:2）、少量的花生四烯酸（C20:4）和很少的亚麻酸（C18:3）。相比之下，80%-90%汇合的 BMSCs 含有可比比例的棕榈酸和油酸，显著更多的花生四烯酸和硬脂酸，很少的亚油酸，并且没有亚麻酸。分化后，与成体脂肪细胞相比，BMSC 来源的脂肪细胞含有可比比例的棕榈酸、更多的硬脂酸和花生四烯酸、更少的油酸、几乎没有亚油酸和检测不到的亚麻酸。这种组成与未分化的 BMSCs 非常相似。分化培养基仅含有棕榈酸和硬脂酸，有痕量的油酸；它不含有必需的多不饱和脂肪酸。因此，BMSC 来源的脂肪细胞的 FFA 组成与成体脂肪细胞不同。BMSC 来源的脂肪细胞具有改变的饱和和单不饱和 FFA 组成，并且缺乏可能直接影响与其脂肪分解/脂肪生成功能相关的信号传导的必需 FFA。
