Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Macromol Biosci. 2012 Feb;12(2):241-50. doi: 10.1002/mabi.201100227. Epub 2011 Oct 25.
A biodegradable electroactive hydrogel (AP-g-GA), aniline pentamer (AP) grafting gelatin (GA), is synthesized by a coupling reaction between the carboxyl group of AP and the amino side group of GA in aqueous solution. The electroactivity of the physical hydrogel is confirmed by UV-vis and CV. The hydrophobic AP changes the hydrogel's porous structure of the natural GA and the gel-time, which is confirmed by the rheological behavior of the AP-g-GA and GA. With an increase in the content of AP, the hydrogel gradually forms a porous structure, from "honeycomb" to "bamboo raft". The porous scaffolds can be crosslinked with 3.5% EDC in 90% ethanol. MTT assays show that the AP-g-GA exhibits reduced cytotoxicity compared to EM AP due to the introduction of the biocompatible GA moiety. The in vitro cell cultures suggest that the AP-g-GA#1 (with 1.9% AP) shows the best biocompatibility and cell adhesion ability.
一种可生物降解的电活性水凝胶（AP-g-GA），即苯胺五聚体（AP）接枝明胶（GA），是通过在水溶液中 AP 的羧基与 GA 的氨基侧基之间的偶联反应合成的。物理水凝胶的电活性通过 UV-vis 和 CV 得到证实。疏水性的 AP 改变了天然 GA 的水凝胶多孔结构和胶凝时间，这通过 AP-g-GA 和 GA 的流变行为得到了证实。随着 AP 含量的增加，水凝胶逐渐形成多孔结构，从“蜂巢”到“竹筏”。多孔支架可以用 3.5%的 EDC 在 90%乙醇中交联。MTT 分析表明，由于引入了生物相容性 GA 部分，AP-g-GA 与 EM AP 相比表现出降低的细胞毒性。体外细胞培养表明，AP-g-GA#1（含 1.9%AP）表现出最佳的生物相容性和细胞黏附能力。
