Centro Integrato di Ricerche, Laboratory of Clinical Microbiology, University Campus Bio-medico, 00128 Rome, Italy.
Diagn Microbiol Infect Dis. 2012 Jan;72(1):20-31. doi: 10.1016/j.diagmicrobio.2011.09.015. Epub 2011 Oct 24.
We performed a comparative evaluation of the Vitek-2 Compact and Phoenix systems for direct identification and antimicrobial susceptibility testing (AST) from positive blood culture bottles in comparison to the standard methods. Overall, 139 monomicrobial blood cultures, comprising 91 Gram-negative and 48 Gram-positive isolates, were studied. Altogether, 100% and 92.3% of the Gram-negative isolates and 75% and 43.75% of the Gram-positive isolates showed concordant identification between the direct and the standard methods with Vitek and Phoenix, respectively. AST categorical agreements of 98.7% and 99% in Gram-negative and of 96.2% and 99.5% in Gram-positive isolates with Vitek and Phoenix, respectively, were observed. In conclusion, direct inoculation procedures for Gram-negative isolates showed an excellent performance with both automated systems, while for identification of Gram-positive isolates they proved to be less reliable, although Vitek provided acceptable results. This approach contributes to reducing the turnaround time to result of blood cultures, with a positive impact on patient care.
我们对 Vitek-2 Compact 和 Phoenix 系统进行了比较评估，以比较标准方法，直接从阳性血培养瓶中进行鉴定和抗菌药物敏感性测试 (AST)。总的来说，研究了 139 株单种血培养物，包括 91 株革兰氏阴性和 48 株革兰氏阳性分离株。Vitek 和 Phoenix 分别与直接和标准方法之间的革兰氏阴性分离株的一致性鉴定率为 100%和 92.3%，革兰氏阳性分离株的一致性鉴定率为 75%和 43.75%。Vitek 和 Phoenix 分别观察到革兰氏阴性和革兰氏阳性分离株的 AST 分类协议的 98.7%和 99%和 96.2%和 99.5%。总之，两种自动化系统对革兰氏阴性分离株的直接接种程序表现出极好的性能，而对于革兰氏阳性分离株的鉴定，它们的可靠性较差，尽管 Vitek 提供了可接受的结果。这种方法有助于缩短血培养的结果周转时间，对患者护理产生积极影响。
