Department of Clinical Laboratory, Kyorin University Hospital, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, Japan.
Diagn Microbiol Infect Dis. 2012 Jun;73(2):129-34. doi: 10.1016/j.diagmicrobio.2012.03.004. Epub 2012 Apr 10.
This study describes a direct inoculation method using the automated BacT/ALERT 3D and the BD Phoenix System in combination for identification and susceptibility testing of isolates from positive blood cultures. Organism identification and susceptibility results were compared with the conventional method for 211 positive aerobic blood cultures. Of 110 Gram-positive cocci (GPCs), 98 (89.1%) isolates were correctly identified to the species level. Of 101 Gram-negative rods (GNRs), 98 (97.0%) isolates were correctly identified to the species level. The overall categorical agreement in antimicrobial susceptibility testing among the 110 GPCs was 92.7%, with 0.04% very major and 0.7% major error rates. The overall categorical agreement among 78 isolates of enterobacteria and 23 isolates of nonfermenters in GNRs was 99.5% and 91.1%, respectively, with no major errors identified. We conclude that, compared with previously reported direct inoculation methods, our method is superior in identification and susceptibility testing of GPCs.
本研究描述了一种使用自动化 BacT/ALERT 3D 和 BD Phoenix 系统直接接种的方法，用于鉴定和药敏试验阳性血培养物中的分离株。将微生物鉴定和药敏试验结果与 211 份阳性需氧血培养的常规方法进行比较。在 110 株革兰阳性球菌（GPC）中，98 株（89.1%）分离株被正确鉴定到种水平。在 101 株革兰阴性杆菌（GNR）中，98 株（97.0%）分离株被正确鉴定到种水平。110 株 GPC 中抗菌药物敏感性试验的总类别一致性为 92.7%，其中 0.04%为非常重大误差，0.7%为重大误差。肠杆菌属 78 株和非发酵菌属 23 株 GNR 中，总体类别一致性分别为 99.5%和 91.1%，未发现重大误差。我们得出结论，与之前报道的直接接种方法相比，我们的方法在 GPC 的鉴定和药敏试验方面更具优势。
